Western Blot (WB)
(Western blot analysis of CFHL1 antibody (C-term) in K562 cell line lysates (35ug/lane). CFHL1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CFHR1 Polyclonal Antibody | anti-CFHR1 antibody
CFHR1, CT (Complement Factor H-related Protein 1, FHR-1, H Factor-like Protein 1, H-Factor-like 1, H36, CFHL, CFHL1, CFHL1P, CFHR1P, FHR1, HFL1, HFL2) (MaxLight 490)
Gene Names
CFHR1; CFHL; FHR1; HFL1; HFL2; CFHL1; H36-1; H36-2; CFHL1P; CFHR1P
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
CFHR1; Polyclonal Antibody; CT (Complement Factor H-related Protein 1; FHR-1; H Factor-like Protein 1; H-Factor-like 1; H36; CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; HFL1; HFL2) (MaxLight 490); anti-CFHR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CFHL1 at ~37kD.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-CFHR1 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a portion of amino acids within aa276-306 from the C-terminal region of human CFHL1 (KLH)
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CFHL1 antibody (C-term) in K562 cell line lysates (35ug/lane). CFHL1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CFHL1 antibody (C-term) in K562 cell line lysates (35ug/lane). CFHL1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CFHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CFHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using CFHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using CFHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CFHR1 antibody
References
1. Grosskinsky, S., PLoS ONE 4(3): E4858 (2009). 2. Siegel, C., J. Biol. Chem. 283(50): 34, 855-34, 863 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,651 Da
NCBI Official Full Name
complement factor H-related protein 1
NCBI Official Synonym Full Names
complement factor H-related 1
NCBI Official Symbol
CFHR1
NCBI Official Synonym Symbols
CFHL; FHR1; HFL1; HFL2; CFHL1; H36-1; H36-2; CFHL1P; CFHR1P
NCBI Protein Information
complement factor H-related protein 1; FHR-1; H factor (complement)-like 1; H factor (complement)-like 2; H-factor-like 1; H36; complement factor H-related 1 pseudogene; h factor-like protein 1
UniProt Protein Name
Complement factor H-related protein 1
Protein Family
UniProt Gene Name
CFHR1
UniProt Synonym Gene Names
CFHL; CFHL1; CFHL1P; CFHR1P; FHR1; HFL1; HFL2; FHR-1; H-factor-like 1
UniProt Entry Name
FHR1_HUMAN
Uniprot Description
CFHR1: Might be involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: extracellular space
Biological Process: complement activation
Disease: Macular Degeneration, Age-related, 1; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 1
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Product Notes
The CFHR1 cfhr1 (Catalog #AAA6470019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CFHR1, CT (Complement Factor H-related Protein 1, FHR-1, H Factor-like Protein 1, H-Factor-like 1, H36, CFHL, CFHL1, CFHL1P, CFHR1P, FHR1, HFL1, HFL2) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CFHR1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 FLISA: 1:1000 IHC: 1:50-1:100 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CFHR1 cfhr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFHR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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