Rabbit CETN3 Polyclonal Antibody | anti-CETN3 antibody

CETN3 Polyclonal Antibody

Cat. Num. AAA9128006

• Gene Names: CETN3; CEN3; CDC31
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: CETN3; Polyclonal Antibody; CETN3 Polyclonal Antibody; anti-CETN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 167

• Applicable Applications for anti-CETN3 antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CETN3
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CETN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using CETN3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using CETN3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using CETN3 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-CETN3 antibody

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 663071182
• NCBI GeneID: 1070
• NCBI Accession #: NP_001284697.1
• NCBI GenBank Nucleotide #: NM_001297768.1
• UniProt Accession #: O15182; Q53YD2; Q9BS23
• Molecular Weight: 20kDa
• NCBI Official Full Name: centrin-3 isoform 3
• NCBI Official Synonym Full Names: centrin, EF-hand protein, 3
• NCBI Official Symbol: CETN3
• NCBI Official Synonym Symbols: CEN3; CDC31
• NCBI Protein Information: centrin-3
• UniProt Protein Name: Centrin-3
• Protein Family: Centrin
• UniProt Gene Name: CETN3
• UniProt Synonym Gene Names: CEN3
• UniProt Entry Name: CETN3_HUMAN

NCBI Description

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

CETN3: Plays a fundamental role in microtubule-organizing center structure and function. Belongs to the centrin family.

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: centriole; centrosome; nucleolus

Molecular Function: calcium ion binding

Biological Process: mitosis; cell division; centrosome cycle