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Western Blot (WB) (Western Blot analysis of Human fetal liver tissue, HT-29 and HUVEC cell, MCF-7 cell and Human hepatocellular carcinoma tissue using CERS4 Polyclonal Antibody at dilution of 1:300)

Rabbit CERS4 Polyclonal Antibody | anti-CERS4 antibody

CERS4 Polyclonal Antibody

Gene Names
CERS4; Trh1; LASS4
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
CERS4; Polyclonal Antibody; CERS4 Polyclonal Antibody; Trh1; LASS4; anti-CERS4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
2.3mg/mL (varies by lot)
Sequence Length
394
Applicable Applications for anti-CERS4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Synthetic peptide of human CERS4
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Human fetal liver tissue, HT-29 and HUVEC cell, MCF-7 cell and Human hepatocellular carcinoma tissue using CERS4 Polyclonal Antibody at dilution of 1:300)

Western Blot (WB) (Western Blot analysis of Human fetal liver tissue, HT-29 and HUVEC cell, MCF-7 cell and Human hepatocellular carcinoma tissue using CERS4 Polyclonal Antibody at dilution of 1:300)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human ovarian cancer using CERS4 Polyclonal Antibody at dilution of 1:35)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human ovarian cancer using CERS4 Polyclonal Antibody at dilution of 1:35)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human prostate cancer using CERS4 Polyclonal Antibody at dilution of 1:35)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human prostate cancer using CERS4 Polyclonal Antibody at dilution of 1:35)
Related Product Information for anti-CERS4 antibody
The LASS (longevity assurance homolog) family members are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1, LASS2, LASS3, LASS4, LASS5 and LASS6) and they are all involved in sphingolipid synthesis. LASS4 is a 394 amino acid endoplasmic reticulum, multi-pass membrane protein. LASS4 increases the levels of long ceramides such as C22:0- and C24:0-ceramides. In cells deficient for CLN9, as observed in neuronal ceroid lipofuscinosis (NCL) or Batten disease, LASS4 can increase ceramide levels and partially correct growth and apoptosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,399 Da
NCBI Official Full Name
ceramide synthase 4
NCBI Official Synonym Full Names
ceramide synthase 4
NCBI Official Symbol
CERS4
NCBI Official Synonym Symbols
Trh1; LASS4
NCBI Protein Information
ceramide synthase 4; LAG1 homolog, ceramide synthase 4; LAG1 longevity assurance homolog 4
UniProt Protein Name
Ceramide synthase 4
Protein Family
UniProt Gene Name
CERS4
UniProt Synonym Gene Names
LASS4; CerS4
UniProt Entry Name
CERS4_HUMAN

Uniprot Description

Lass4: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; integral to membrane

Molecular Function: DNA binding; sphingosine N-acyltransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; ceramide biosynthetic process

Research Articles on CERS4

Similar Products

Product Notes

The CERS4 cers4 (Catalog #AAA2522039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CERS4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CERS4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CERS4 cers4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CERS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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