Western Blot/Blotting
(Western blot analysis using Ceramide Kinase antibody (X1705P) on 30 ng of recombinant ceramide kinase. Antibody used at 2 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.)
Rabbit anti-Human Ceramide Kinase (rProtein-Full Length) Polyclonal Antibody | anti-CERK antibody
Ceramide Kinase (rProtein-Full Length)
Western Blot/Blotting
(Western blot analysis using Ceramide Kinase antibody (X1705P) on 30 ng of recombinant ceramide kinase. Antibody used at 2 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.)
Western Blot/Blotting
(Western blot analysis using Ceramide Kinase antibody (X1705P) on 30 ng of recombinant ceramide kinase. Antibody used at 2 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.)
NCBI and Uniprot Product Information
NCBI Description
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. Ref.8
Catalytic activity: ATP + ceramide = ADP + ceramide 1-phosphate.
Cofactor: Calcium.Magnesium.
Enzyme regulation: Inhibited by sulfatide. Ref.8
Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein Ref.8.
Tissue specificity: High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
Sequence similarities: Contains 1 DAGKc domain.
Biophysicochemical propertiespH dependence:Optimum pH is 6.0-7.5.
Research Articles on CERK
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Product Notes
The CERK cerk (Catalog #AAA395565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ceramide Kinase (rProtein-Full Length) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ceramide Kinase (rProtein-Full Length) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the CERK cerk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ceramide Kinase (rProtein-Full Length), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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