Rabbit CEP70 Polyclonal Antibody | anti-CEP70 antibody

CEP70, ID (CEP70, BITE, Centrosomal protein of 70kD, p10-binding protein) (AP)

Cat. Num. AAA6285194

• Gene Names: CEP70; BITE
• Reactivity: Hamster, Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CEP70; Polyclonal Antibody; ID (CEP70; BITE; Centrosomal protein of 70kD; p10-binding protein) (AP); p10-binding protein; anti-CEP70 antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CEP70 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CEP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the Central region of human CEP70.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(CEP70 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CEP70 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(CEP70 Antibody (Center) western blot analysis in WiDr,CHO,A549,U251 cell line lysates (35ug/lane).This demonstrates the CEP70 antibody detected the CEP70 protein (arrow).)

Flow Cytometry (FC/FACS)

(CEP70 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CEP70 antibody

Antibodies; Centrosomal Proteins

References

Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Lim, J., et al. Cell 125(4):801-814(2006). Petretti, C., et al. EMBO Rep. 7(4):418-424(2006). Andersen, J.S., et al. Nature 426(6966):570-574(2003). Casenghi, M., et al. Dev. Cell 5(1):113-125(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 50658082
• NCBI GeneID: 80321
• NCBI Accession #: NP_077817.2
• NCBI GenBank Nucleotide #: NM_024491.2
• UniProt Accession #: Q8NHQ1; Q96B31; Q9H2C3; Q9H2Z1; Q9H940; B7Z5I8; B7Z7E8; D3DNE9; F5GZX8
• Molecular Weight: 69,752 Da
• NCBI Official Full Name: centrosomal protein of 70 kDa
• NCBI Official Synonym Full Names: centrosomal protein 70kDa
• NCBI Official Symbol: CEP70
• NCBI Official Synonym Symbols: BITE
• NCBI Protein Information: centrosomal protein of 70 kDa; p10-binding protein
• UniProt Protein Name: Centrosomal protein of 70 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP70
• UniProt Synonym Gene Names: BITE; Cep70
• UniProt Entry Name: CEP70_HUMAN

Uniprot Description

CEP70: Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: nucleoplasm; centrosome; nuclear membrane; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle