Goat CENTD2/ARAP1 Polyclonal Antibody | anti-ARAP1 antibody
Goat anti-CENTD2/ARAP1 Antibody
Immunohistochemistry: This antibody showed staining concentrated at the ruffles over lamellipodia and focal complexes but not at focal adhesions in NIH3T3 cells.
Western Blot: This antibody showed a 150KDa in NIH3T3 lysates.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
CENTD2: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: cytoplasmic vesicle; cytosol; Golgi apparatus; plasma membrane; trans-Golgi network
Molecular Function: GTPase activator activity; metal ion binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; type 1 angiotensin receptor binding
Biological Process: actin filament reorganization during cell cycle; negative regulation of stress fiber formation; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of receptor recycling; regulation of cell motility; regulation of cell shape; regulation of small GTPase mediated signal transduction; signal transduction
Research Articles on ARAP1
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Product Notes
The ARAP1 arap1 (Catalog #AAA420453) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CENTD2/ARAP1 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CENTD2/ARAP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: This antibody showed staining concentrated at the ruffles over lamellipodia and focal complexes but not at focal adhesions in NIH3T3 cells. Western Blot: This antibody showed a 150KDa in NIH3T3 lysates. Researchers should empirically determine the suitability of the ARAP1 arap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AFTADPLSLL RNV. It is sometimes possible for the material contained within the vial of "CENTD2/ARAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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