Western Blot (WB)
(Western blot analysis of CENPK expression in HEK293T (A) whole cell lysates.)
Rabbit anti-Human, Mouse CENPK Polyclonal Antibody | anti-CENPK antibody
Anti-CENPK Antibody
IF/ICC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of CENPK expression in HEK293T (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CENPK expression in HEK293T (A) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of CENPK staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of CENPK staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
Uniprot Description
CENPK: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL. Belongs to the CENPK family.
Chromosomal Location of Human Ortholog: 5q12.3
Cellular Component: nucleoplasm; cytosol
Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle
Research Articles on CENPK
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Product Notes
The CENPK cenpk (Catalog #AAA8306250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CENPK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CENPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CENPK cenpk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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