Rabbit CELSR3 Polyclonal Antibody | anti-CELSR3 antibody

CELSR3 Polyclonal Antibody

Cat. Num. AAA3007367

• Gene Names: CELSR3; FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CELSR3; Polyclonal Antibody; CELSR3 Polyclonal Antibody; Cadherin EGF LAG seven-pass G-type receptor 3; Cadherin family member 11; Epidermal growth factor-like protein 1; EGF-like protein 1; Flamingo homolog 1; hFmi1; Multiple epidermal growth factor-like domains protein 2; Multiple EGF-like domains protein 2; CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; anti-CELSR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CELSR3 pAb detects endogenous levels of CELSR3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 3312

• Applicable Applications for anti-CELSR3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human CELSR3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CELSR3 pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug))

Related Product Information for anti-CELSR3 antibody

Drosophila Flamingo is a seven pass transmembrane cadherin that is necessary for dendritic patterning and axon guidance. Flamingo mammalian homologs play similar roles in neuronal development, during which they play an important role in cell-cell signaling. Cadherin EGF LAG seven pass G-type receptors (CELSRs) are multi-pass membrane proteins that belong to the G protein-coupled receptor family of proteins. Silencing CELSR2 gene expression results in signficant simplification of dendritic arbors of cortical pyramidal neurons and Purkinje neurons, which may be due to branch retraction. In mouse, CELSR1, CELSR2 and CELSR3 are expressed in the nervous system at early developmental stages, and show expression patterns in the developing CNS. CELSR2 is distributed at intercellular boundaries in the whisker and on processes of neuronal cells such as hippocampal pyramidal cells, Purkinje cells and olfactory neurons.

NCBI and Uniprot Product Information

• NCBI GI #: 145309304
• NCBI GeneID: 1951
• NCBI Accession #: NP_001398.2
• NCBI GenBank Nucleotide #: NP_001398.2
• UniProt Accession #: Q9NYQ7
• Molecular Weight: ~ 358kDa
• NCBI Official Full Name: cadherin EGF LAG seven-pass G-type receptor 3
• NCBI Official Synonym Full Names: cadherin EGF LAG seven-pass G-type receptor 3
• NCBI Official Symbol: CELSR3
• NCBI Official Synonym Symbols: FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
• NCBI Protein Information: cadherin EGF LAG seven-pass G-type receptor 3
• UniProt Protein Name: Cadherin EGF LAG seven-pass G-type receptor 3
• Protein Family: Cadherin EGF LAG seven-pass G-type receptor
• UniProt Gene Name: CELSR3
• UniProt Synonym Gene Names: CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; EGF-like protein 1; hFmi1
• UniProt Entry Name: CELR3_HUMAN

NCBI Description

This gene belongs to the flamingo subfamily, which is included in the cadherin superfamily. The flamingo cadherins consist of nonclassic-type cadherins that do not interact with catenins. They are plasma membrane proteins containing seven epidermal growth factor-like repeats, nine cadherin domains and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic feature of their subfamily. The encoded protein may be involved in the regulation of contact-dependent neurite growth and may play a role in tumor formation. [provided by RefSeq, Jun 2013]

Uniprot Description

CELSR3: Receptor that may have an important role in cell/cell signaling during nervous system formation. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of protein localization; axonal fasciculation; neuron migration; cilium biogenesis; homophilic cell adhesion