Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '22131'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.82 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '22131' and pd.language_id = 1
Query
Database
1.52 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '22131'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
2.75 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '22131'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '22131' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '22131'
⇄⧉products_description => string (240) "Principle of the Assay: Under the condition of weak acidity, NEFA react with...
$value['products_description']
Principle of the Assay: Under the condition of weak acidity, NEFA react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
⇄⧉products_description => string (240) "Principle of the Assay: Under the condition of weak acidity, NEFA react with...
$value->a['products_description']
Principle of the Assay: Under the condition of weak acidity, NEFA react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
⇄⧉products_description => string (240) "Principle of the Assay: Under the condition of weak acidity, NEFA react with...
$value->d['products_description']
Principle of the Assay: Under the condition of weak acidity, NEFA react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
⇄products_references => string (3) "N/A"
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_mim => string (3) "N/A"
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⇄sp_interactions => string (3) "N/A"
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⇄⧉public fields_below -> array (3)
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⇄0 => string (31) "Related Product Information for"
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of D...
$value[0]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of Defa5. No significant cross-reactivity or interference between Defa5 and analogues was observed.
⇄purity => string (3) "N/A"
$value[0]['_source']['purity']
⇄form => string (3) "N/A"
$value[0]['_source']['form']
⇄concentration => string (3) "N/A"
$value[0]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[0]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉etc_term1 => string (152) "Assay Type||Sandwich!!Samples||Serum, plasma, tissue homogenates and other b...
$value[0]['_source']['etc_term1']
Assay Type||Sandwich!!Samples||Serum, plasma, tissue homogenates and other biological fluids!!Detection Range||15.625-1000pg/ml!!Sensitivity||9.375pg/ml
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[0]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄⧉search_terms => string (543) "aaa17683 mouse this assay has high sensitivity and excellent specificity for...
$value[0]['_source']['search_terms']
aaa17683 mouse this assay has high sensitivity and excellent specificity for detection of defa5 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17683_sc elisa kit defensin alpha 5 paneth cell specific related cryptdin def5 hd 10,071 da hd5 20 94 def5_human 50057850 aat68886.1 q01523 q3knv2 a0jdy6 600472 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 15.625 1000pg ml 9.375pg intra precision cv alpha5 hd520
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of D...
$value[1]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of DEFA5. No significant cross-reactivity or interference between DEFA5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DEFA5 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[1]['_source']['purity']
⇄form => string (3) "N/A"
$value[1]['_source']['form']
⇄concentration => string (3) "N/A"
$value[1]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1399) "Intended Uses: This DEFA5 ELISA kit is a 1.5 hour solid-phase ELISA designed...
$value[1]['_source']['products_description']
Intended Uses: This DEFA5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DEFA5. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: DEFA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DEFA5 antibody and an DEFA5-HRP conjugate. The assay sample and buffer are incubated together with DEFA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFA5 concentration since DEFA5 from samples and DEFA5-HRP conjugate compete for the anti-DEFA5 antibody binding site. Since the number of sites is limited, as more sites are DEFA5cupied by DEFA5 from the sample, fewer sites are left to bind DEFA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFA5 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (747) "aaa16334 human this assay has high sensitivity and excellent specificity for...
$value[1]['_source']['search_terms']
aaa16334 human this assay has high sensitivity and excellent specificity for detection of defa5 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16334_sc elisa kit defensin alpha 5 paneth cell specific defalpha def5 hd hd5 20 94 preproprotein 10,071 da cleaved into following 4 chains:hd5 23 29 56 63 def5_human 50057850 aat68886.1 q01523 q3knv2 a0jdy6 600472 immunology samples serum plasma culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml alpha5 hd520 following4 chains:hd523 competitive0.1
<b>Storage:</b><br>Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C for one month.<br>Aliquot and store at -80 degree C for 12 months.<br><br><b>Stability Test:</b><br>The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
⇄app_tested => string (57) "Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)."
$value[2]['_source']['app_tested']
⇄app_notes => string (75) "(May be suitable for use in other assays to be determined by the end user.)"
⇄⧉etc_term2 => string (210) "Residues||Glu20~Arg94!!Tags||N-terminal His Tag!!Subcellular Location||Secre...
$value[2]['_source']['etc_term2']
Residues||Glu20~Arg94!!Tags||N-terminal His Tag!!Subcellular Location||Secreted, Exosome!!Predicted isoelectric point||5.9!!Usage||Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
⇄⧉search_terms => string (806) "aaa20221 e.coli homo sapiens human > 90 pbs ph7.4 containing 0.01 skl 1mm dt...
$value[2]['_source']['search_terms']
aaa20221 e.coli homo sapiens human > 90 pbs ph7.4 containing 0.01 skl 1mm dtt 5 trehalose and proclin300 positive control immunogen sds page western blot wb may be suitable for use in other assays to determined by the end user sequence information aaa20221_seq gene sequencing extract aaa20221_td2 aaa20221_sds3 recombinant protein defensin alpha paneth cell specific defa5 preproprotein def5 hd hd5 20 94 predicted molecular mass 14.8kda accurate 15kda as reducing conditions def5_human 10337585 np_066290.1 q01523 nm_021010.1 q3knv2 a0jdy6 600472 source prokaryotic expression residues glu20~arg94 tags n terminal his tag subcellular location secreted exosome traits freeze dried powder isoelectric point 5.9 usage reconstitute a concentration of 0.1 1.0 mg ml do not vortex >90 dtt5 hd520 point5.9 of0.1
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
$value[3]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human DEFA3. No significant cross-reactivity or interference between human DEFA3 and analogues was observed.
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[3]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[3]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[3]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉ncbi_pathways => string (241) "Alpha-defensins Pathway||530758!!Defensins Pathway||530757!!Immune System Pa...
$value[3]['_source']['ncbi_pathways']
Alpha-defensins Pathway||530758!!Defensins Pathway||530757!!Immune System Pathway||106386!!Innate Immune System Pathway||106387!!Transcriptional Misregulation In Cancer Pathway||523016!!Transcriptional Misregulation In Cancer Pathway||522987
⇄⧉search_terms => string (555) "aaa15851 human this assay has high sensitivity and excellent specificity for...
$value[3]['_source']['search_terms']
aaa15851 human this assay has high sensitivity and excellent specificity for detection of defa3 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15851_td elisa kit defensin alpha 3 neutrophil specific def3 hnp hnp3 hp peptide preproprotein hp3 10,245 da 2 hp2 def3_human 4885179 np_005208.1 p59666 nm_005217.3 p11479 q14125 604522 samples serum plasma tissue homogenates type quantitative sandwich range 1.87 ng ml 120 < 0.47 intra precision within an cv alpha3 da2 ml120
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of m...
$value[4]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of mouse DEFA4. No significant cross-reactivity or interference between mouse DEFA4 and analogues was observed.
⇄purity => string (3) "N/A"
$value[4]['_source']['purity']
⇄form => string (3) "N/A"
$value[4]['_source']['form']
⇄concentration => string (3) "N/A"
$value[4]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[4]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[4]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[4]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (709) "aaa18044 mouse this assay has high sensitivity and excellent specificity for...
$value[4]['_source']['search_terms']
aaa18044 mouse this assay has high sensitivity and excellent specificity for detection of defa4 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18044_td elisa kit defensin alpha 4 corticostatin neutrophil def4 hnp hp hp4 mgc120099 mgc138296 preproprotein crp4 defa28 defcr4 28 related cryptdin 10,272 da defa4_mouse 6753626 np_034169.1 p28311 nm_010039.1 q9qzl4 a3kpc1 samples serum plasma tissue homogenates cell lysates type quantitative sandwich range 0.312 ng ml 20 < 0.078 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in alpha4 defcr428 ml20
<b>Storage:</b><br>Avoid repeated freeze/thaw cycles.<br>Store at 4 degree C for frequent use.<br>Aliquot and store at -20 degree C for 24 months.<br><br><b>Stability Test:</b><br>thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western blotting: 0.01-2ug/mL<br>I mmunohistochemistry: 5-30ug/mL<br>I mmunocytochemistry: 5-30ug/mL<br>Optimal working dilutions must be determined by end user.
⇄⧉testing_protocols => string (1385) "IHC (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Glioma ...
$value[5]['_source']['testing_protocols']
IHC (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Glioma Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistchemistry)||DAB staining on IHC-P;<br>Samples: Human Colorectal cancer Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Cerebrum Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Prostate cancer Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody;<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Pancreas Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Prostate Tissue;<br>Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot)||Western Blot:<br>Sample: Recombinant DEFa3, Human.||AAA20180_WB.jpg
Alpha-defensins Pathway||1269266!!Antimicrobial Peptides Pathway||1457777!!Defensins Pathway||1269265!!Immune System Pathway||1269170!!Innate Immune System Pathway||1269203!!Transcriptional Misregulation In Cancer Pathway||523016!!Transcriptional Misregulation In Cancer Pathway||522987
⇄⧉search_terms => string (1053) "aaa20180 rabbit human polyclonal antigen specific affinity chromatography fo...
$value[5]['_source']['search_terms']
aaa20180 rabbit human polyclonal antigen specific affinity chromatography followed by protein a supplied as solution form in 0.01m pbs ph7.4 containing 0.05 proclin 300 50 glycerol the antibody is raised against defa3 it has been selected for its ability to recognize immunohistochemical staining andwestern blotting wb ihc icc ip western 0.5 2ug ml immunohistochemistry 5 20ug immunocytochemistry optimal working dilutions must be determined end user blot sample recombinant aaa20180_wb dab on p samples pancreas tissue aaa20180_ihc target fused with n terminal his tag and sequence listed below mghhhhhhsgsef e plqaradeva aapeqiaadi pevvvslawd eslapkhpgs rknmdcycri paciagerry gtciyqgrlw afcc defensin alpha 3 neutrophil preproprotein hp3 def3 hnp3 hp hnp 10,245 da 2 hp2 4885179 np_005208.1 p59666 nm_005217.3 p11479 q14125 m21131 mrna source preparation traits liquid cross reactivity immunogen pro21~cys94 expressed e.coli conjugated apc version of this item also available catalog #mbs2073998 proclin300 western0.5 immunohistochemistry5 alpha3 da2
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
$value[6]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human DEFA1. No significant cross-reactivity or interference between human DEFA1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[6]['_source']['purity']
⇄form => string (3) "N/A"
$value[6]['_source']['form']
⇄concentration => string (3) "N/A"
$value[6]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[6]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[6]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[6]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Alpha-defensins Pathway||530758!!Cellular Roles Of Anthrax Toxin Pathway||138076!!Defensins Pathway||530757!!Immune System Pathway||106386!!Innate Immune System Pathway||106387
⇄⧉search_terms => string (611) "aaa15540 human this assay has high sensitivity and excellent specificity for...
$value[6]['_source']['search_terms']
aaa15540 human this assay has high sensitivity and excellent specificity for detection of defa1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15540_td elisa kit defensin alpha 1 def1 defa2 hnp hp mgc138393 mrs otthump00000196017 myeloid related sequence 2 neutrophil 1b defa1b hp1 10,201 da mrsdefa1b hp2 def1_human 124248516 np_001035965.1 p59665 nm_001042500.1 p11479 q14125 q6ezf6 125220 samples serum plasma urine type quantitative sandwich range 0.625 ng ml 40 < 0.156 intra precision within an cv alpha1 sequence2 ml40
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of D...
$value[7]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of DEFB2. No significant cross-reactivity or interference between DEFB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DEFB2 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
$value[7]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1403) "Principle of the Assay: DEFB2 ELISA kit applies the competitive enzyme immun...
$value[7]['_source']['products_description']
Principle of the Assay: DEFB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DEFB2 antibody and an DEFB2-HRP conjugate. The assay sample and buffer are incubated together with DEFB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFB2 concentration since DEFB2 from samples and DEFB2-HRP conjugate compete for the anti-DEFB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFB2 from the sample, fewer sites are left to bind DEFB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFB2 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This DEFB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine DEFB2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of D...
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This assay has high sensitivity and excellent specificity for detection of DEFB1. No significant cross-reactivity or interference between DEFB1 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
$value[8]['_source']['form']
⇄concentration => string (3) "N/A"
$value[8]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
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Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[8]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (206) "Inflammation||65!!Immune System Diseases||46!!Necrosis||35!!Lung Diseases||3...
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Inflammation||65!!Immune System Diseases||46!!Necrosis||35!!Lung Diseases||33!!Carcinoma||24!!Fibrosis||18!!Disease Models, Animal||12!!Kidney Diseases||9!!Precancerous Conditions||5!!Prostatic Neoplasms||4
⇄⧉search_terms => string (530) "aaa17457 human this assay has high sensitivity and excellent specificity for...
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aaa17457 human this assay has high sensitivity and excellent specificity for detection of defb1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17457_sc elisa kit beta defensin 1 bd1 hbd1 bd hbd preproprotein defb defb101 7,420 da defb1_human 4885181 np_005209.1 p60022 nm_005218.3 q09753 602056 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 31.25 2000pg ml 18.75pg intra precision cv defensin1
⇄⧉products_description => string (1353) "Intended Uses: This CDC5L ELISA kit is a 1.5 hour solid-phase ELISA designed...
$value[9]['_source']['products_description']
Intended Uses: This CDC5L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCDC5L. This ELISA kit for research use only!<br><br>Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (482) "aaa16736 canine typical testing data standard curve for reference only aaa16...
$value[9]['_source']['search_terms']
aaa16736 canine typical testing data standard curve for reference only aaa16736_sc elisa kit cadherin 5 cdh5 preproprotein type 2 vascular endothelium 7b4 cd144 antigen ve endothelial specific epithelium 74,499 da cd_antigen cadh5_human 166362713 np_001786.2 p33151 nm_001795.3 q4vai5 q4vai6 601120 signal transduction samples serum plasma cell culture supernatants body fluid and tissue homogenate assay quantitative competitive sensitivity 0.1 ng ml cadherin5 type2 sensitivity0.1
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of D...
$value[10]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of Defb1. No significant cross-reactivity or interference between Defb1 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
$value[10]['_source']['form']
⇄concentration => string (3) "N/A"
$value[10]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[10]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[10]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄products_references => string (3) "N/A"
$value[10]['_source']['products_references']
⇄⧉products_related_diseases => string (206) "Inflammation||65!!Immune System Diseases||46!!Necrosis||35!!Lung Diseases||3...
⇄⧉search_terms => string (528) "aaa17677 mouse this assay has high sensitivity and excellent specificity for...
$value[10]['_source']['search_terms']
aaa17677 mouse this assay has high sensitivity and excellent specificity for detection of defb1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17677_sc elisa kit beta defensin 1 preproprotein bd1 hbd1 defb defb101 7,420 da bd hbd defb1_human 4885181 np_005209.1 p60022 nm_005218.3 q09753 602056 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.781 50ng ml 0.469ng intra precision cv defensin1
⇄⧉specificity => string (205) "This assay has high sensitivity and excellent specificity for detection of h...
$value[11]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human beta-defensins 1. No significant cross-reactivity or interference between human beta-defensins 1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[11]['_source']['purity']
⇄form => string (3) "N/A"
$value[11]['_source']['form']
⇄concentration => string (3) "N/A"
$value[11]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[11]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (779) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[11]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (540) "aaa15556 human this assay has high sensitivity and excellent specificity for...
$value[11]['_source']['search_terms']
aaa15556 human this assay has high sensitivity and excellent specificity for detection of beta defensins 1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15556_td elisa kit defensin bd1 defb defb101 hbd1 mgc51822 defb1 preproprotein bd 7,420 da hbd defb1_human 4885181 np_005209.1 p60022 nm_005218.3 q09753 602056 samples serum plasma tissue homogenates type quantitative sandwich range 3.12 pg ml 200 < 0.78 intra precision within an cv defensins1 ml200
⇄⧉specificity => string (173) "This assay has high sensitivity and excellent specificity for detection of C...
$value[12]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of COL4a5. No significant cross-reactivity or interference between COL4a5 and analogues was observed.
⇄purity => string (3) "N/A"
$value[12]['_source']['purity']
⇄form => string (3) "N/A"
$value[12]['_source']['form']
⇄concentration => string (3) "N/A"
$value[12]['_source']['concentration']
⇄⧉storage_stability => string (475) "The stability of kit is determined by the loss rate of activity. The loss ra...
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Double-antibody Sandwich!!Samples||Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids!!Detection Range||3.12-200ng/mL!!Sensitivity||1.17ng/mL
⇄⧉etc_term2 => string (418) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4a5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4a5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄products_price => string (6) "0.0000"
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⇄products_weight => string (4) "5.00"
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⇄products_status => boolean true
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⇄products_tax_class_id => string (1) "1"
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⇄manufacturers_id => string (4) "2000"
$value[12]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[12]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[12]['_source']['language_id']
⇄products_name => string (33) "Collagen Type IV Alpha 5 (COL4a5)"
$value[12]['_source']['products_name']
⇄products_name_oem => string (49) "Human Collagen Type IV Alpha 5 (COL4a5) ELISA Kit"
$value[12]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[12]['_source']['products_name_syn']
⇄products_gene_name => string (6) "COL4a5"
$value[12]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[12]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1033) "Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantit...
$value[12]['_source']['products_description']
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4a5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.<br><br>Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL4a5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4a5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4a5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL4a5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (686) "aaa21093 human this assay has high sensitivity and excellent specificity for...
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aaa21093 human this assay has high sensitivity and excellent specificity for detection of col4a5 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa21093_sc elisa kit collagen type iv alpha 5 metabolic pathway developmental science nutrition metabolism samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids quantitative sandwich range 3.12 200ng ml < 1.12ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 alpha5 an3 tested20 plates8
⇄⧉products_description => string (786) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[13]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antigen Sandwich ELISA technique. The pre-coated antigen is a related-Canine anti-dsDNA IgG-specific antigen, while the detection antigen is another antigen. Samples are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (339) "aaa22631 canine no cross reaction with other factors typical testing data st...
$value[13]['_source']['search_terms']
aaa22631 canine no cross reaction with other factors typical testing data standard curve for reference only aaa22631_sc elisa kit dsdna igg specific anti samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision <= 8 inter 12 range20 <=8 inter12
⇄reactivity => string (61) "Human, Mouse, Rat<br>No cross reactivity with other proteins."
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⇄specificity => string (3) "N/A"
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⇄purity => string (3) "N/A"
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⇄form => string (11) "Lyophilized"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (202) "Store at -20 degree C for one year. After reconstitution, at 4 degree C for ...
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Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
⇄app_tested => string (58) "ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)"
⇄⧉testing_protocols => string (4865) "IHC (Immunohistchemistry)||Figure 6. IHC analysis of Integrin alpha 5 using ...
$value[14]['_source']['testing_protocols']
IHC (Immunohistchemistry)||Figure 6. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19156_IHC6.jpg!!IHC (Immunohistochemistry)||Figure 5. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Integrin alpha 5 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19156_IHC5.jpg!!IHC (Immunohistochemistry)||Figure 4. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Integrin alpha 5 was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19156_IHC4.jpg!!IHC (Immunohistochemistry)||Figure 3. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Integrin alpha 5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19156_IHC3.jpg!!IHC (Immunohistochemistry)||Figure 2. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Integrin alpha 5 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19156_IHC2.jpg!!WB (Western Blot)||Figure 1. Western blot analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>Lane 1: human Hela cell lysate,<br>Lane 2: human HepG2 cell lysate,<br>Lane 3: rat liver tissue lysate,<br>Lane 4: mouse liver tissue lysate.<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin alpha 5 at approximately 150KD. The expected band size for Integrin alpha 5 is at 114KD.||AAA19156_WB.jpg
⇄⧉etc_term1 => string (195) "Immunogen||E Coli-derived human Integrin alpha 5 recombinant protein (Positi...
$value[14]['_source']['etc_term1']
Immunogen||E Coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).!!Subcellular Localization||Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion.
⇄⧉etc_term2 => string (169) "Reconstitution||Add 0.2ml of distilled water will yield a concentration of 5...
$value[14]['_source']['etc_term2']
Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.!!Contents||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
⇄⧉products_name_syn => string (192) "Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor su...
$value[14]['_source']['products_name_syn']
Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; ITGA5; FNRA
⇄products_gene_name => string (5) "ITGA5"
$value[14]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[14]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1123) "Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein tha...
$value[14]['_source']['products_description']
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.<br>Protein Function: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5: ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed: 18635536, PubMed: 25398877). ITGA5: ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed: 12807887, PubMed: 17158881).
⇄⧉search_terms => string (1687) "aaa19156 rabbit human mouse rat no cross reactivity with other proteins poly...
$value[14]['_source']['search_terms']
aaa19156 rabbit human mouse rat no cross reactivity with other proteins polyclonal lyophilized elisa eia immunohistochemistry ihc western blot wb 0.1 0.5mug ml paraffin embedded section 0.5 1mug direct figure 1 analysis of integrin alpha 5 using anti antibody electrophoresis was performed on a 20 sds page gel at 70v stacking 90v resolving for 2 3 hours the sample well each lane loaded 50ug under reducing conditions hela cell lysate hepg2 liver tissue 4 after were transferred to nitrocellulose membrane 150ma 50 90 minutes blocked non fat milk tbs 1.5 hour rt incubated antigen affinity purified 0.5ug overnight degree c then washed tween times and probed goat igg hrp secondary dilution 1:10000 signal is developed an enhanced chemiluminescent detection ecl kit tanon 5200 system specific band detected approximately 150kd expected size 114kd aaa19156_wb in colon cancer heat mediated retrieval citrate buffer ph6 epitope solution mins 10 serum 1ug biotinylated used as 30 37 strepavidin biotin complex sabc dab chromogen aaa19156_ihc2 lung aaa19156_ihc3 cardiac muscle aaa19156_ihc4 aaa19156_ihc5 6 placenta aaa19156_ihc6 picoband cd49 like family member e fibronectin receptor subunit f vla cd49e heavy chain light itga5 fnra vla5a 114,536 da cd_antigen 1017029567 np_002196.4 p08648 nm_002205.4 q96ha5 x06256 mrna immunogen coli derived recombinant protein position f42 a263 subcellular localization single pass type i junction focal adhesion reconstitution add 0.2ml distilled water will yield concentration 500ug contents vial contains 4mg trehalose 0.9mg nacl 0.2mg na2hpo4 0.05mg nan3 wb0.1 section0.5 figure1 alpha5 a20 for2 tissue4 150ma50 tbs1.5 mins10 as30 aaa19156_ihc56
⇄⧉specificity => string (189) "This assay has high sensitivity and excellent specificity for detection of h...
$value[15]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human DEFB104A. No significant cross-reactivity or interference between human DEFB104A and analogues was observed.
⇄purity => string (3) "N/A"
$value[15]['_source']['purity']
⇄form => string (3) "N/A"
$value[15]['_source']['form']
⇄concentration => string (3) "N/A"
$value[15]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[15]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Samples||Serum, plasma, cell culture supernates, tissue homogenates!!Assay Type||Sandwich!!Detection Range||31.25 pg/ml -2000 pg/ml.!!Sensitivity||The minimum detectable dose of human DEFB104A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[15]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (747) "Principle of the assay: This assay employs the quantitative sandwich enzyme ...
$value[15]['_source']['products_description']
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFB104A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB104A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB104A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB104A bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (930) "aaa18118 human this assay has high sensitivity and excellent specificity for...
$value[15]['_source']['search_terms']
aaa18118 human this assay has high sensitivity and excellent specificity for detection of defb104a no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18118_td elisa kit defensin beta 104a 104 bd 4 defb defb104 defb4 mgc118942 mgc118944 mgc118945 hbd 104b defb104b 8,526 da defb4<br ><strong>defb104b< strong> d104a_human 103485137 np_001035792.1 q8wtq1 nm_001040702.1 q496i2 q496i3 q496i4 samples serum plasma cell culture supernates tissue homogenates type sandwich range 31.25 pg ml 2000 the minimum detectable dose is typically less than 7.8 lower limit lld defined as lowest protein concentration that could be differentiated from zero it determined mean o.d value 20 replicates added by their three deviations intra precision within an cv <8 known were tested twenty times on one plate to assess inter assays <10 in 104a104 bd4 than7.8 value20
⇄⧉etc_term1 => string (233) "Samples||human serum, plasma or cell culture supernatant and organizations i...
$value[16]['_source']['etc_term1']
Samples||human serum, plasma or cell culture supernatant and organizations in the natural and recombinant 5-LO concentration.!!Assay Type||Sandwich (Quantitative)!!Detection Range||10 ng/ml-0.156 ng/ml!!Sensitivity||Up to 0.05 ng/ml.
⇄⧉search_terms => string (575) "aaa12510 human no cross reaction with other factors typical testing data sta...
$value[16]['_source']['search_terms']
aaa12510 human no cross reaction with other factors typical testing data standard curve for reference only aaa12510_td elisa kit 5 lipoxygenase lo partial arachidonate alox5 5lpg log5 lox leukotriene a4 synthase arachidonic acid alpha 10 isoform delta 13 p10 56,291 da lox5_human 187167 aaa63212.1 p09917 q5jq14 b7zls0 e5fpy5 e5fpy7 e5fpy8 152390 samples serum plasma or cell culture supernatant and organizations in the natural recombinant concentration assay type sandwich quantitative detection range ng ml 0.156 sensitivity up to 0.05 intra precision kit5 alpha10 delta13
⇄⧉specificity => string (205) "This assay has high sensitivity and excellent specificity for detection of h...
$value[17]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human beta-defensins 3. No significant cross-reactivity or interference between human beta-defensins 3 and analogues was observed.
⇄purity => string (3) "N/A"
$value[17]['_source']['purity']
⇄form => string (3) "N/A"
$value[17]['_source']['form']
⇄concentration => string (3) "N/A"
$value[17]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[17]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[17]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (779) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[17]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (632) "aaa15028 human this assay has high sensitivity and excellent specificity for...
$value[17]['_source']['search_terms']
aaa15028 human this assay has high sensitivity and excellent specificity for detection of beta defensins 3 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15028_td elisa kit defensin 103a defb103 defb3 hbd hbd3 hbp hbp3 defb103a 103 bd defb like protein 7,697 da bd3 defb3defb103b d103a_human 125656159 np_001075020.1 p81534 nm_001081551.3 q8nfg6 q9npf6 606611 samples serum plasma cell culture supernates urine tissue homogenates type quantitative sandwich range 125 pg ml 8000 < 31.25 intra precision within an cv defensins3 defb103a103 range125
⇄⧉etc_term1 => string (140) "Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative...
$value[18]['_source']['etc_term1']
Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||0.31-20ng/mL!!Sensitivity||0.19ng/mL
⇄⧉etc_term2 => string (372) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value[18]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human 5-NT were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human 5-NT were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (1213) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
$value[18]['_source']['products_description']
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human 5-NT concentrations in serum, plasma and other biological fluids.<br><br>Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human 5-NT. You can calculate the concentration of Human 5-NT in the samples by comparing the OD of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value[18]['_source']['products_references']
⇄⧉products_related_diseases => string (196) "Inflammation||17!!Necrosis||9!!Breast Neoplasms||9!!Nervous System Diseases|...
⇄⧉search_terms => string (704) "aaa21913 human this kit recognizes 5 nt in samples no significant cross reac...
$value[18]['_source']['search_terms']
aaa21913 human this kit recognizes 5 nt in samples no significant cross reactivity or interference between and analogues was observed typical testing data standard curve for reference only aaa21913_td elisa nucleotidase 5' isoform 2 preproprotein ecto cd73 nt5e en nt5 nte ent e5nt calja purine prime 57,949 da cd_antigen 5ntd_human 325651886 np_001191742.1 p21589 nm_001204813.1 o75520 q5w116 b3kqi8 129190 serum plasma other biological fluids assay type quantitative sandwich detection range 0.31 20ng ml sensitivity 0.19ng intra precision within an 3 with low mid high level were tested 20 times on one plate respectively inter assays different plates replicates each recognizes5 isoform2 an3 tested20
⇄⧉etc_term1 => string (104) "Samples||Serum, plasma and other biological fluids!!Detection Range||0.16-10...
$value[19]['_source']['etc_term1']
Samples||Serum, plasma and other biological fluids!!Detection Range||0.16-10ng/mL!!Sensitivity||0.1ng/mL
⇄⧉etc_term2 => string (370) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value[19]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MOG were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MOG were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (154) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
$value[19]['_source']['products_description']
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse MOG concentrations in serum, plasma and other biological fluids.
⇄products_references => string (3) "N/A"
$value[19]['_source']['products_references']
⇄⧉products_related_diseases => string (238) "Nervous System Diseases||1390!!Demyelinating Diseases||1273!!Brain Diseases|...
⇄⧉search_terms => string (704) "aaa21622 mouse this kit recognizes mog in samples no significant cross react...
$value[19]['_source']['search_terms']
aaa21622 mouse this kit recognizes mog in samples no significant cross reactivity or interference between and analogues was observed typical testing data standard curve for reference only aaa21622_td elisa myelin oligodendrocyte glycoprotein protein btn6 btnl11 mogig2 nrclp7 ig alub alpha 5 33,528 da mog_human 23270927 aah35938.1 q16653 o00713 o00714 o00715 q13054 q13055 a6ndr4 a6nnj9 a8my31 b0uzr9 e9pgf0 f8w9d5 159465 serum plasma other biological fluids assay type quantitative sandwich detection range 0.16 10ng ml sensitivity 0.1ng intra precision within an 3 with low mid high level were tested 20 times on one plate respectively inter assays different plates replicates each alpha5 an3 tested20
