Rabbit anti-Human CDX2 Polyclonal Antibody | anti-CDX2 antibody

CDX2, NT (CDX2, CDX3, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2) (PE)

Cat. Num. AAA6284841

• Gene Names: CDX2; CDX3; CDX-3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDX2; Polyclonal Antibody; NT (CDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2) (PE); Caudal-type homeobox protein 2; anti-CDX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CDX2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~38 amino acids from the N-terminal region of human CDX2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDX2(arrow) using rabbit polyclonal CDX2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDX2 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(The anti-CDX2 N-term Pab is used in Western blot to detect CDX2 in placenta tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CDX2 antibody

The caudal type homeo box transcription factors 1 (CDX1) and 2 (CDX2) are candidates for directing intestinal development, differentiation, and maintenance of the intestinal phenotype. CDX1 and CDX2 expression is widely present in the human intestinal and colonic mucosae, but not in the gastric mucosa, suggesting a possible role in the terminal differentiation of the intestine. Increased CDX2 expression is associated with chronic atrophic gastritis. Detectable expression of CDX2 precedes expression of CDX1 during the progression of intestinal metaplasia, thus expression of CDX2 may trigger the initiation and development of intestinal metaplasia. Markedly reduced or absent CDX2 expression was noted by immunohistochemistry in 13 of 15 (87%) large cell minimally differentiated carcinomas (LCMDCs), whereas only 1 of the 25 (4%) differentiated adenocarcinomas (DACs) showed reduced CDX2 expression. Thus, a significant decrease in human CDX1 and/or CDX2 expression may be associated with colorectal tumorigenesis.

Product Categories/Family for anti-CDX2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Phillips, R.W., et al., Am. J. Surg. Pathol. 27(11):1442-1447 (2003). Bai, Y.Q., et al., Oncogene 22(39):7942-7949 (2003). Yamamoto, H., et al., Biochem. Biophys. Res. Commun. 300(4):813-818 (2003). Eda, A., et al., J. Gastroenterol. 37(2):94-100 (2002). Moucadel, V., et al., Biochem. Biophys. Res. Commun. 297(3):607-615 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 372624392
• NCBI GeneID: 1045
• NCBI Accession #: NP_001256.3
• NCBI GenBank Nucleotide #: NM_001265.4
• UniProt Accession #: Q99626; O00503; Q5VTU7; Q969L8; Q9UD92
• Molecular Weight: 33,520 Da
• NCBI Official Full Name: homeobox protein CDX-2
• NCBI Official Synonym Full Names: caudal type homeobox 2
• NCBI Official Symbol: CDX2
• NCBI Official Synonym Symbols: CDX3; CDX-3
• NCBI Protein Information: homeobox protein CDX-2; caudal-type homeobox protein 2; caudal type homeobox transcription factor 2; caudal type homeo box transcription factor 2
• UniProt Protein Name: Homeobox protein CDX-2
• Protein Family: Homeobox protein
• UniProt Gene Name: CDX2
• UniProt Synonym Gene Names: CDX3
• UniProt Entry Name: CDX2_HUMAN

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]

Uniprot Description

CDX2: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Belongs to the Caudal homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; condensed nuclear chromosome; transcriptional repressor complex

Molecular Function: double-stranded DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of epithelial cell polarity; anterior/posterior pattern formation; blood vessel development; organ morphogenesis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; endosome to lysosome transport; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; trophectodermal cell differentiation