Rabbit anti-Human, Mouse CDK8 Polyclonal Antibody | anti-CDK8 antibody

CDK8 Rabbit Polyclonal

Cat. Num. AAA767795

• Gene Names: CDK8; K35
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CDK8; Polyclonal Antibody; CDK8 Rabbit Polyclonal; anti-CDK8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 464

• Applicable Applications for anti-CDK8 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Cyclin-dependent kinase 8
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer using MBS767795 (CDK8 antibody) at dilution of 1:50)

Western Blot (WB)

(NIH/3T3 cells were subjected to SDS PAGE followed by western blot with MBS767795 (CDK8 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 1000491
• NCBI GeneID: 1024
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5
• Molecular Weight: 53,156 Da
• NCBI Official Full Name: CDK8 protein kinase
• NCBI Official Synonym Full Names: cyclin dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35
• NCBI Protein Information: cyclin-dependent kinase 8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK8
• UniProt Entry Name: CDK8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK8: a protein kinase of the CDK family. Along with cyclin C forms part of the RNA polymerase II holoenzyme complex. Phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Regulates transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Kinase, protein; Cell cycle regulation; EC 2.7.11.23; Protein kinase, CMGC; CMGC group; CDK family; CDK8 subfamily

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; nucleus; Srb-mediator complex

Molecular Function: ATP binding; cyclin-dependent protein kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cell cycle; transcription initiation from RNA polymerase II promoter