Rabbit anti-Human Cdk7 Polyclonal Antibody | anti-Cdk7 antibody

Cdk7 Polyclonal Antibody

Cat. Num. AAA3012462

• Gene Names: CDK7; CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Cdk7; Polyclonal Antibody; Cdk7 Polyclonal Antibody; p39 Mo15; CDK7; 39 kDa protein kinase; CAK; CAK1; Cdk activating kinase; CDK-activating kinase 1; CDK-activating kinase; cdk7; CDKN7; Cell division protein kinase 7; Cyclin dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog; Xenopus laevis; cdk-activating kinase); Cyclin-dependent kinase 7; HCAK; Homolog of Xenopus MO15 Cdk activating kinase; Kinase subunit of CAK; MO15; Xenopus; homolog of; P39 Mo15; p39MO15; Serine threonine kinase Stk1; Serine/threonine protein kinase 1; Serine/threonine protein kinase MO15; STK1; TFIIH basal transcription factor complex kinase subunit; anti-Cdk7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cdk7 polyclonal antibody detects endogenous levels of Cdk7 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 346

• Applicable Applications for anti-Cdk7 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; ICC: 1:100-1:500; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunocytochemistry (ICC)

(ICC staining Cdk7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Cdk7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Cdk7 antibody

Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity.

NCBI and Uniprot Product Information

• NCBI GI #: 4502743
• NCBI GeneID: 1022
• NCBI Accession #: NP_001790.1
• NCBI GenBank Nucleotide #: NP_001790.1
• UniProt Accession #: P50613
• Molecular Weight: 39kDa
• NCBI Official Full Name: cyclin-dependent kinase 7 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase 7
• NCBI Official Symbol: CDK7
• NCBI Official Synonym Symbols: CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• NCBI Protein Information: cyclin-dependent kinase 7
• UniProt Protein Name: Cyclin-dependent kinase 7
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK7
• UniProt Synonym Gene Names: CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
• UniProt Entry Name: CDK7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Protein kinase, CMGC; EC 2.7.11.23; CMGC group; CDK family; CDK7 subfamily; CDK/CDK7 subfamily

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: nucleoplasm; mitochondrion; holo TFIIH complex; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; androgen receptor binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; G2/M transition of mitotic cell cycle; cell cycle arrest; transcription initiation from RNA polymerase II promoter; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; cell proliferation; nucleotide-excision repair; cell division; RNA elongation from RNA polymerase II promoter; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle