Rabbit CDK6 Polyclonal Antibody | anti-CDK6 antibody

CDK6 Polyclonal Antibody

Cat. Num. AAA3012460

• Gene Names: CDK6; MCPH12; PLSTIRE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Peptide affinity purified
• Synonyms: CDK6; Polyclonal Antibody; CDK6 Polyclonal Antibody; CDK 6; Cell division protein kinase 6; Crk 2; Crk2; Cyclin dependent kinase 6; Cyclin-dependent kinase 6; MGC59692; p40; PLSTIRE; Serine/threonine protein kinase PLSTIRE; Serine/threonine-protein kinase PLSTIRE; STQTL11; anti-CDK6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDK6 polyclonal antibody detects endogenous levels of CDK6 protein.
• Purity/Purification: Peptide affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 326

• Applicable Applications for anti-CDK6 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB: 1:2,000; ICC: 1:200; IHC: 1:200
• Immunogen: peptide
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2000 dilution. Positive control: Lane 1: K562 Lane 2: COS-1)

Immunocytochemistry (ICC)

(ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-CDK6 antibody

CDK6 protein contains a catalytic core composed of a serine/threonine domain.This protein also contains an ATP-binding pocket, inhibitory and activating phosphorylation sites, a PSTAIRE-like cyclin-binding domain and an activating T-loop motif. CDK6 is involved in a positive feedback loop that activates transcription factors through a reaction cascade. CDK6 is important for the control of G1 to S phase transition. However, in recent years, new evidence proved that the presence of CDK6 is not essential for proliferation in every cell type, the cell cycle has a complex circuitry of regulation and the role of CDK6 might be more important in certain cell types than in others, where CDK4 or CDK2 can act as protein kinases compensating its role.

NCBI and Uniprot Product Information

• NCBI GI #: 223718134
• NCBI GeneID: 1021
• NCBI Accession #: NP_001138778.1
• NCBI GenBank Nucleotide #: NP_001138778.1
• UniProt Accession #: Q00534
• Molecular Weight: 37kDa
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: MCPH12; PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK6
• UniProt Synonym Gene Names: CDKN6
• UniProt Entry Name: CDK6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]