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Western Blot (WB)
(Anti-CDK4 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:33 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human CDK4 Polyclonal Antibody | anti-CDK4 antibody
Anti-CDK4 Antibody, Rabbit Polyclonal
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CDK4; Polyclonal Antibody; Anti-CDK4 Antibody; Rabbit Polyclonal; CDK4 Antibody; Rabbit PAb; Antigen Affinity Purified; cyclin-dependent kinase 4; Anti-CMM3 Antibody; Anti-PSK-J3 Antibody; anti-CDK4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CDK4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CDK4 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human CDK4 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CDK4 (rh CDK4). CDK4 specific IgG was purified by Human CDK4 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CDK4 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:33 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CDK4 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:33 kDa(We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human CDK4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human CDK4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunofluorescence (IF)
(Immunofluorescence staining of CDK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDK4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of CDK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDK4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Immunoprecipitation (IP)
(CDK4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg k562 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-CDK4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Immunoprecipitation (IP)
(CDK4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg k562 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-CDK4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Western Blot (WB)
(Anti-CDK4 rabbit polyclonal antibody at 1:500 dilution Lane A: CDK4 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:33 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CDK4 rabbit polyclonal antibody at 1:500 dilution Lane A: CDK4 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:33 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CDK4 antibody
CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product. CDK4 is the ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. CDK4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma.
Product Categories/Family for anti-CDK4 antibody
References
Stepanova L, et al. (1996) Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4. Genes Dev. 10(12):1491-502.
Lamphere L, et al. (1997) Interaction between Cdc37 and Cdk4 in human cells. Oncogene. 14(16): 1999-2004.
Dai K, et al. (1996) Physical interaction of mammalian CDC37 with CDK4. J Biol Chem. 271(36): 22030-4.
Sugimoto M, et al. (1999) Regulation of CDK4 activity by a novel CDK4-binding protein, p34SEI-1. Genes Dev. 13(22):3027-33.
Lamphere L, et al. (1997) Interaction between Cdc37 and Cdk4 in human cells. Oncogene. 14(16): 1999-2004.
Dai K, et al. (1996) Physical interaction of mammalian CDC37 with CDK4. J Biol Chem. 271(36): 22030-4.
Sugimoto M, et al. (1999) Regulation of CDK4 activity by a novel CDK4-binding protein, p34SEI-1. Genes Dev. 13(22):3027-33.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34kDa
NCBI Official Full Name
cyclin-dependent kinase 4
NCBI Official Synonym Full Names
cyclin-dependent kinase 4
NCBI Official Symbol
CDK4
NCBI Official Synonym Symbols
CMM3; PSK-J3
NCBI Protein Information
cyclin-dependent kinase 4; cell division protein kinase 4
UniProt Protein Name
Cyclin-dependent kinase 4
Protein Family
UniProt Gene Name
CDK4
UniProt Entry Name
CDK4_HUMAN
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Product Notes
The CDK4 cdk4 (Catalog #AAA8103738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CDK4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 ICC/IF: 1:300-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the CDK4 cdk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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