CDK1 Polyclonal Antibody | anti-CDK1 antibody

Anti-CDK1 Antibody

Cat. Num. AAA177904

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: CDK1; Polyclonal Antibody; Anti-CDK1 Antibody; Cyclin-dependent kinase 1; Cdc 2; Cdc2; CDC28A; CDK 1; CDK1_HUMAN; CDKN1; CELL CYCLE CONTROLLER CDC2; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell division protein kinase 1; Cell Divsion Cycle 2 Protein; Cyclin Dependent Kinase 1; DKFZp686L20222; MGC111195; p34 Cdk1; p34 protein kinase; P34CDC2; cyclin-dependent kinase 1; anti-CDK1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 297

• Applicable Applications for anti-CDK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- CDK1 Picoband antibody, MBS177904, Western blottingAll lanes: Anti CDK1 (MBS177904) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugLane 6: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 34KDObserved bind size: 34KD )

Immunohistochemistry (IHC)

(Anti- CDK1 Picoband antibody, MBS177904, IHC(P)IHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti- CDK1 Picoband antibody, MBS177904, IHC(P)IHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti- CDK1 Picoband antibody, MBS177904, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-CDK1 antibody

Description: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 1(CDK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

References

1. Draetta, G.; Piwnica-Worms, H.; Morrison, D.; Druker, B.; Roberts, T.; Beach, D.: Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Nazarenko, S. A.; Ostroverhova, N. V.; Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991. 3. Spurr, N. K.; Goodfellow, P. N.; Nurse, P.; Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987.

NCBI and Uniprot Product Information

• NCBI GI #: 4502709
• NCBI GeneID: 983
• NCBI Accession #: NP_001777.1
• NCBI GenBank Nucleotide #: NM_001786.4
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497
• Molecular Weight: 27,503 Da
• NCBI Official Full Name: cyclin-dependent kinase 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2; CDC28A; CDKN1; P34CDC2; CDK1
• UniProt Entry Name: CDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Cell cycle regulation; Protein kinase, CMGC; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: centrosome; cytoplasm; cytosol; membrane; midbody; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; apoptosis; cell aging; cell division; cell migration; centrosome cycle; chromosome condensation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication; epithelial cell differentiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; G2/M transition of mitotic cell cycle; histone phosphorylation; microtubule cytoskeleton organization and biogenesis; mitosis; mitotic cell cycle G2/M transition DNA damage checkpoint; mitotic nuclear envelope disassembly; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; organ regeneration; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cardiac muscle cell proliferation; positive regulation of DNA replication; positive regulation of mitotic cell cycle; positive regulation of protein import into nucleus, translocation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of embryonic development; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to activity; response to amine stimulus; response to axon injury; response to cadmium ion; response to copper ion; response to drug; response to ethanol; response to organic cyclic substance; response to toxin; ventricular cardiac muscle cell development