Rabbit cdk1, aa42-57 Polyclonal Antibody | anti-CDK1 antibody

cdk1, aa42-57 (cdc2, PSTAIR, Cyclin-dependent Kinase 1)

Cat. Num. AAA620792

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by antigen affinity chromatography.
• Synonyms: cdk1; aa42-57; Polyclonal Antibody; aa42-57 (cdc2; PSTAIR; Cyclin-dependent Kinase 1); Anti -cdk1; anti-CDK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human cdk1/cdc2 kinase (34kD). Species crossreactivity
• Purity/Purification: Affinity Purified; Purified by antigen affinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-CDK1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: Western Blot: 0.5-2ug/ml detected cdc2 from human A431, mouse 3T3 and rat L6 RIPA cell lysates.; Immunoprecipitation: 4ug Immunoprecipitates cdc2 from 500ug of A431 RIPA lysates.
• Immunogen: Synthetic peptide corresponding to aa 42-57, EGVPSTAIREISLLKE-GGC, of human cdc2 kinase. The immunogen is identical in a wide range of species.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunofluorescence (IF)

(Confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor®488), and DAPI (Blue, Nucleus). Staining shows positive nuclear staining.)

Testing Data

(A431 cell lysate probed with MBS620792 at ug/ml)

Related Product Information for anti-CDK1 antibody

Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60 src are substrates for cdc2.

Product Categories/Family for anti-CDK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 334302921
• NCBI GeneID: 983
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497; Q5H9N4
• Molecular Weight: 34,095 Da
• NCBI Official Full Name: Cyclin-dependent kinase 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222
• NCBI Protein Information: cyclin-dependent kinase 1; OTTHUMP00000019659; OTTHUMP00000019660; OTTHUMP00000223862; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2
• UniProt Entry Name: CDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 2.7.11.23; Cell cycle regulation; EC 2.7.11.22; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; centrosome; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; nucleus; cytosol

Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: regulation of Schwann cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; response to toxin; histone phosphorylation; regulation of embryonic development; ventricular cardiac muscle cell development; centrosome cycle; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; response to activity; toll-like receptor 9 signaling pathway; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; response to amine stimulus; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; peptidyl-threonine phosphorylation; microtubule cytoskeleton organization and biogenesis; DNA repair; MyD88-dependent toll-like receptor signaling pathway; G1/S-specific transcription in mitotic cell cycle; peptidyl-serine phosphorylation; response to cadmium ion; response to copper ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication