Rabbit anti-Human CDH4 Polyclonal Antibody | anti-CDH4 antibody

CDH4, NT (CDH4, Cadherin-4, Retinal cadherin) (PE)

Cat. Num. AAA6284203

• Gene Names: CDH4; CAD4; RCAD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDH4; Polyclonal Antibody; NT (CDH4; Cadherin-4; Retinal cadherin) (PE); Retinal cadherin; anti-CDH4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDH4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CDH4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 175-203 in the N-terminal region of human CDH4, UniProt Accession #P55283. Species sequence homology: mouse and rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS6001229 in HepG2 cell line lysates (35ug/lane). CDH4(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6001229 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunofluorescence analysis of MBS6001229 with paraffin-embedded human brain tissue. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS6001229 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDH4 antibody

CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.

Product Categories/Family for anti-CDH4 antibody

Antibodies; Cadherin

References

1. Miotto, E., Cancer Res. 64 (22), 8156-8159 (2004). Johnson, E., J. Biol. Chem. 279 (30), 31041-31049 (2004). 2. Kitagawa, M., Biochem. Biophys. Res. Commun. 271 (2), 358-363 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 356640221
• NCBI GeneID: 1002
• NCBI Accession #: NP_001239267.1
• NCBI GenBank Nucleotide #: NM_001252338.1
• UniProt Accession #: P55283; Q2M208; Q5VZ44; Q9BZ05; B3KWB8; G3V1P8
• Molecular Weight: 100,281 Da
• NCBI Official Full Name: cadherin-4 isoform 2
• NCBI Official Synonym Full Names: cadherin 4, type 1, R-cadherin (retinal)
• NCBI Official Symbol: CDH4
• NCBI Official Synonym Symbols: CAD4; RCAD
• NCBI Protein Information: cadherin-4; R-CAD; R-cadherin; retinal cadherin; cadherin 4, type 1, preproprotein
• UniProt Protein Name: Cadherin-4
• UniProt Gene Name: CDH4
• UniProt Synonym Gene Names: R-CAD; R-cadherin
• UniProt Entry Name: CADH4_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CDH4: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. Expressed mainly in brain but also found in other tissues.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: heterophilic cell adhesion; axon guidance; intercellular junction assembly and maintenance; positive regulation of axon extension; cell adhesion; homophilic cell adhesion