Rabbit anti-Human CDCA8 Polyclonal Antibody | anti-CDCA8 antibody

CDCA8, NT (CDCA8, PESCRG3, Borealin, Dasra-B, Cell division cycle-associated protein 8, Pluripotent embryonic stem cell-related gene 3 protein) (APC)

Cat. Num. AAA6284040

• Gene Names: CDCA8; BOR; DasraB; MESRGP; BOREALIN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CDCA8; Polyclonal Antibody; NT (CDCA8; PESCRG3; Borealin; Dasra-B; Cell division cycle-associated protein 8; Pluripotent embryonic stem cell-related gene 3 protein) (APC); Pluripotent embryonic stem cell-related gene 3 protein; anti-CDCA8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDCA8 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human CDCA8.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDCA8 (arrow) using rabbit polyclonal CDCA8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDCA8 gene.)

Immunohistochemistry (IHC)

(CDCA8 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDCA8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CDCA8 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDCA8 antibody

CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle (Gassmann et al., 2004 [PubMed 15249581]).

Product Categories/Family for anti-CDCA8 antibody

Antibodies; Cell Division Cycle (CDC)

References

Bourhis, E., et al. Biochemistry 48(29):6783-6793(2009). Zhang, Q., et al. Mol. Biol. Rep. 36(3):603-609(2009). Klein, U.R., et al. Mol. Biol. Cell 20(1):410-418(2009). Xia, F., et al. Mol. Cell. Biol. 28(17):5299-5311(2008). Jelluma, N., et al. Cell 132(2):233-246(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 8922438
• NCBI GeneID: 55143
• NCBI Accession #: NP_060571.1
• NCBI GenBank Nucleotide #: NM_018101.3
• UniProt Accession #: Q53HL2
• NCBI Official Full Name: borealin
• NCBI Official Synonym Full Names: cell division cycle associated 8
• NCBI Official Symbol: CDCA8
• NCBI Official Synonym Symbols: BOR; DasraB; MESRGP; BOREALIN
• NCBI Protein Information: borealin
• UniProt Protein Name: Borealin
• UniProt Gene Name: CDCA8
• UniProt Synonym Gene Names: PESCRG3; hDasra-B
• UniProt Entry Name: BOREA_HUMAN

NCBI Description

This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]

Uniprot Description

Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.

Protein type: Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis