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SDS-Page (Gel: 12%SDS-PAGE lysate: 40 ¦Œg, Lane 1-2: 293T and LOVO cell lysates, Primary antibody: 46447£¨CDCA4 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Rabbit anti-Human CDCA4 Polyclonal Antibody | anti-CDCA4 antibody

CDCA4 Antibody

Gene Names
CDCA4; HEPP; SEI-3/HEPP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
CDCA4; Polyclonal Antibody; CDCA4 Antibody; HEPP; SEI-3/HEPP; anti-CDCA4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of total CDCA4 protein.
Purity/Purification
Antigen affinity purification
Form/Format
In pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.7mg/ml (varies by lot)
Sequence Length
1550
Applicable Applications for anti-CDCA4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:5000
IHC: 1: 50-300
Immunogen
Full length fusion protein of human CDCA4
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C.

SDS-Page

(Gel: 12%SDS-PAGE lysate: 40 ¦Œg, Lane 1-2: 293T and LOVO cell lysates, Primary antibody: 46447£¨CDCA4 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

SDS-Page (Gel: 12%SDS-PAGE lysate: 40 ¦Œg, Lane 1-2: 293T and LOVO cell lysates, Primary antibody: 46447£¨CDCA4 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 46447(CDCA4 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200))

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 46447(CDCA4 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200))
Related Product Information for anti-CDCA4 antibody
This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1.
Product Categories/Family for anti-CDCA4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26 kDa
NCBI Official Full Name
Homo sapiens cell division cycle associated 4, mRNA
NCBI Official Synonym Full Names
cell division cycle associated 4
NCBI Official Symbol
CDCA4
NCBI Official Synonym Symbols
HEPP; SEI-3/HEPP
NCBI Protein Information
cell division cycle-associated protein 4
UniProt Protein Name
Cell division cycle-associated protein 4
UniProt Gene Name
CDCA4
UniProt Synonym Gene Names
HEPP

NCBI Description

This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]

Uniprot Description

May participate in the regulation of cell proliferation through the E2F/RB pathway. May be involved in molecular regulation of hematopoietic stem cells and progenitor cell lineage commitment and differentiation ().

Research Articles on CDCA4

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Product Notes

The CDCA4 cdca4 (Catalog #AAA9429594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDCA4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDCA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1: 50-300. Researchers should empirically determine the suitability of the CDCA4 cdca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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