Rabbit anti-Human CDCA4 Polyclonal Antibody | anti-CDCA4 antibody

CDCA4 Rabbit pAb

Cat. Num. AAA4758165

• Gene Names: CDCA4; HEPP; SEI-3/HEPP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CDCA4; Polyclonal Antibody; CDCA4 Rabbit pAb; HEPP; SEI-3/HEPP; anti-CDCA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-CDCA4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000
• Immunogen: Full length fusion protein
• Conjugation: Unconjugated
• Molecular Function: This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1.
• Modification: Unmodified
• Cell Position: Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T and LOVO cell lysates, Primary antibody: 824493(CDCA4 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 824493(CDCA4 Antibody) at dilution 1/70. (Original magnification: ×200))

Product Categories/Family for anti-CDCA4 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 33874431
• NCBI GeneID: 55038
• UniProt Accession #: Q9BXL8; Q8TB18; Q9NWK7
• Molecular Weight: 26,114 Da
• NCBI Official Full Name: Homo sapiens cell division cycle associated 4, mRNA
• NCBI Official Synonym Full Names: cell division cycle associated 4
• NCBI Official Symbol: CDCA4
• NCBI Official Synonym Symbols: HEPP; SEI-3/HEPP
• NCBI Protein Information: cell division cycle-associated protein 4
• UniProt Protein Name: Cell division cycle-associated protein 4
• Protein Family: Cell division cycle-associated protein
• UniProt Gene Name: CDCA4
• UniProt Synonym Gene Names: HEPP
• UniProt Entry Name: CDCA4_HUMAN

NCBI Description

This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]

Uniprot Description

CDCA4: May participate in the regulation of cell proliferation through the E2F/RB pathway. May be involved in molecular regulation of hematopoietic stem cells and progenitor cell lineage commitment and differentiation.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: nucleus

Molecular Function: protein binding