Rabbit anti-Human CDC73 Polyclonal Antibody | anti-CDC73 antibody

CDC73, ID (CDC73, C1orf28, HRPT2, Parafibromin, Cell division cycle protein 73 homolog, Hyperparathyroidism 2 protein)

Cat. Num. AAA649546

• Gene Names: CDC73; HYX; HPTJT; HRPT2; C1orf28
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CDC73; Polyclonal Antibody; ID (CDC73; C1orf28; HRPT2; Parafibromin; Cell division cycle protein 73 homolog; Hyperparathyroidism 2 protein); Anti -CDC73; anti-CDC73 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CDC73 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50; Immunofluorescence: 1:10-50
• Immunogen: CDC73 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139~169 amino acids from the Center region of human CDC73.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDC73 Antibody (Center) in 293 cell line lysates (35ug/lane). CDC73 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CDC73 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CDC73 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CDC73 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Related Product Information for anti-CDC73 antibody

CDC73 encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin.

Product Categories/Family for anti-CDC73 antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 39644616
• NCBI GeneID: 79577
• UniProt Accession #: Q6P1J9; Q6PK51; Q96A07; Q9H245; Q9H5L7; A6NLZ8; B2RBR2
• Molecular Weight: 60,577 Da
• NCBI Official Full Name: CDC73 protein, partial
• NCBI Official Synonym Full Names: cell division cycle 73
• NCBI Official Symbol: CDC73
• NCBI Official Synonym Symbols: HYX; HPTJT; HRPT2; C1orf28
• NCBI Protein Information: parafibromin; hyperparathyroidism 2 protein; cell division cycle protein 73 homolog; Paf1/RNA polymerase II complex component; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog
• UniProt Protein Name: Parafibromin
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC73
• UniProt Synonym Gene Names: C1orf28; HRPT2
• UniProt Entry Name: CDC73_HUMAN

NCBI Description

This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma. [provided by RefSeq, Jul 2009]

Uniprot Description

Function: Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors. Ref.7 Ref.8 Ref.9 Ref.13 Ref.14 Ref.17 Ref.18 Ref.19 Ref.20 Ref.23

Subunit structure: Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with POLR2A, CPSF1, CPSF4, CSTF2, KMT2A/MLL1 and CTNNB1. Interacts with a Set1-like complex that has histone methyltransferase activity and methylates histone H3. Found in a complex with BCL9L or BCL9, CDC73, CTNNB1 and PYGO1 indicative for the participation in a nuclear Wnt signaling complex. Ref.7 Ref.8 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20

Subcellular location: Nucleus Ref.7 Ref.8 Ref.9.

Tissue specificity: Found in adrenal and parathyroid glands, kidney and heart. Ref.9

Involvement in disease: Familial isolated hyperparathyroidism (FIHP) [MIM:145000]: Autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7 Ref.11 Ref.24 Ref.26 Ref.31Hyperparathyroidism-jaw tumor syndrome (HPT-JT) [MIM:145001]: Autosomal dominant, multiple neoplasia syndrome primarily characterized by hyperparathyroidism due to parathyroid tumors. Thirty percent of individuals with HPT-JT may also develop ossifying fibromas, primarily of the mandible and maxilla, which are distinct from the brown tumors associated with severe hyperparathyroidism. Kidney lesions may also occur in HPT-JT as bilateral cysts, renal hamartomas or Wilms tumors.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.25 Ref.26Parathyroid carcinoma (PRTC) [MIM:608266]: These cancers characteristically result in more profound clinical manifestations of hyperparathyroidism than do parathyroid adenomas, the most frequent cause of primary hyperparathyroidism. Early en bloc resection of the primary tumor is the only curative treatment.Note: The gene represented in this entry is involved in disease pathogenesis. Ref.12

Sequence similarities: Belongs to the CDC73 family.

Sequence caution: The sequence AAH07325.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 300.The sequence BAB15608.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.