Rabbit CDC45L Polyclonal Antibody | anti-CDC45L antibody

CDC45L Rabbit Polyclonal

Cat. Num. AAA767766

• Gene Names: CDC45; CDC45L; CDC45L2; PORC-PI-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CDC45L; Polyclonal Antibody; CDC45L Rabbit Polyclonal; anti-CDC45L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 566

• Applicable Applications for anti-CDC45L antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Immunogen: CDC45 cell division cycle 45-like (S. cerevisiae)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue slide using MBS767766 (CDC45L Antibody) at dilution of 1:50)

Testing Data

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Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS767766 (CDC45L antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 4502713
• NCBI GeneID: 8318
• NCBI Accession #: NP_003495.1
• NCBI GenBank Nucleotide #: NM_003504.4
• Molecular Weight: 68,768 Da
• NCBI Official Full Name: cell division control protein 45 homolog isoform 2
• NCBI Official Synonym Full Names: cell division cycle 45
• NCBI Official Symbol: CDC45
• NCBI Official Synonym Symbols: CDC45L; CDC45L2; PORC-PI-1
• NCBI Protein Information: cell division control protein 45 homolog
• UniProt Protein Name: Cell division control protein 45 homolog
• UniProt Gene Name: CDC45
• UniProt Synonym Gene Names: CDC45L; CDC45L2
• UniProt Entry Name: CDC45_HUMAN

NCBI Description

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus

Molecular Function: 3'-5' DNA helicase activity; chromatin binding; DNA replication origin binding; protein binding; single-stranded DNA binding

Biological Process: DNA duplex unwinding; DNA replication; DNA replication checkpoint; DNA replication initiation; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; pre-replicative complex assembly; regulation of chromatin silencing at telomere