Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit CDC26 Polyclonal Antibody | anti-CDC26 antibody
CDC26 Antibody
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using CDC26 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using CDC26 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse lung, using CDC26 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 20 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse lung, using CDC26 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 20 kDa.)
Subunit Structure: V-shaped homodimer. Interacts with CDC16. The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.
Similarity: Belongs to the CDC26 family.
NCBI and Uniprot Product Information
Predicted: 10 kDa
NCBI Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC26: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex. Belongs to the CDC26 family.
Chromosomal Location of Human Ortholog: 9q32
Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol
Molecular Function: protein binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
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Product Notes
The CDC26 cdc26 (Catalog #AAA9611642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC26 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CDC26 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CDC26 cdc26 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC26, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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