Rabbit anti-Human CDC25A Polyclonal Antibody | anti-CDC25A antibody

CDC25A, Phosphorylated (Thr506) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A) (Azide free) (HRP)

Cat. Num. AAA6468405

• Gene Names: CDC25A; CDC25A2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CDC25A; Polyclonal Antibody; Phosphorylated (Thr506) (M-phase Inducer Phosphatase 1; Dual Specificity Phosphatase Cdc25A) (Azide free) (HRP); anti-CDC25A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDC25A phosphorylated at Thr506.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDC25A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr506 of human Cdc25A (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Phospho-CDC25A-T507 Pab is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CDC25A antibody

Antibodies; Enzymes; Phosphatase

References

1. Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). 2. Nemoto, K., et al., Prostate 58(1):95-102 (2004). 3. Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). 4. Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). 5. Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 42490758
• NCBI GeneID: 993
• NCBI Accession #: NP_001780
• NCBI GenBank Nucleotide #: NM_001789.2
• UniProt Accession #: P30304; Q8IZH5; Q96IL3; Q9H2F2
• Molecular Weight: 63.2 kDa (560aa)
• NCBI Official Full Name: M-phase inducer phosphatase 1 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25A
• NCBI Official Symbol: CDC25A
• NCBI Official Synonym Symbols: CDC25A2
• NCBI Protein Information: M-phase inducer phosphatase 1
• UniProt Protein Name: M-phase inducer phosphatase 1
• Protein Family: M-phase inducer phosphatase
• UniProt Gene Name: CDC25A
• UniProt Entry Name: MPIP1_HUMAN

NCBI Description

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: mitosis; cell proliferation; response to radiation; regulation of cell cycle; cell division; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle