Western Blot (WB)
(Western blot analysis of CDC20 Antibody (N-term) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CDC20 Polyclonal Antibody | anti-CDC20 antibody
CDC20, NT (CDC20, Cell division cycle protein 20 homolog, p55CDC)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of CDC20 Antibody (N-term) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CDC20 Antibody (N-term) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(CDC20 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDC20 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.)
Western Blot (WB)
(Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.)
NCBI and Uniprot Product Information
NCBI Description
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.
Protein type: Ubiquitin conjugating system; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: nucleoplasm; spindle pole; centrosome; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; spindle; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein binding; enzyme binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; regulation of dendrite development; regulation of meiosis; protein ubiquitination; cell cycle; anaphase-promoting complex activation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Research Articles on CDC20
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Product Notes
The CDC20 cdc20 (Catalog #AAA6004346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC20, NT (CDC20, Cell division cycle protein 20 homolog, p55CDC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CDC20 cdc20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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