Rabbit anti-Human CD85h Polyclonal Antibody | anti-CD85h antibody

Anti-CD85h Antibody

Cat. Num. AAA8305776

• Gene Names: LILRA2; ILT1; LIR7; CD85H; LIR-7
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: CD85h; Polyclonal Antibody; Anti-CD85h Antibody; ILT1; LIR7; Leukocyte immunoglobulin-like receptor subfamily A member 2; CD85 antigen-like family member H; Immunoglobulin-like transcript 1; ILT-1; Leukocyte immunoglobulin-like receptor 7; LIR-7; anti-CD85h antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of CD85h protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-CD85h antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD85h. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of CD85h expression in SHSY5Y (A) whole cell lysates.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Immunofluorescent analysis of CD85h staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Related Product Information for anti-CD85h antibody

Rabbit polyclonal antibody to CD85h

NCBI and Uniprot Product Information

• NCBI GI #: 195972797
• NCBI GeneID: 11027
• NCBI Accession #: NP_001124389.1
• NCBI GenBank Nucleotide #: NM_001130917.1
• UniProt Accession #: Q8N149; O75020
• Molecular Weight: 52,992 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily A member 2 isoform a
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2
• NCBI Official Symbol: LILRA2
• NCBI Official Synonym Symbols: ILT1; LIR7; CD85H; LIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily A member 2; ILT-1; immunoglobulin-like transcript 1; CD85 antigen-like family member H; leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily A member 2
• UniProt Gene Name: LILRA2
• UniProt Synonym Gene Names: ILT1; LIR7; ILT-1; LIR-7
• UniProt Entry Name: LIRA2_HUMAN

NCBI Description

This gene encodes a member of the leukocyte Ig-like receptor (LIR) family of immunoreceptors that are expressed predominantly on monocytes and B cells, and at lower levels on dendritic cells and natural killer cells. The LIR family comprises of activating (subfamily A) and inhibitory (subfamily B) cell-surface receptors. The LIR encoded by this gene was shown to be an activating receptor for eosinophils that elicited the release of cytotoxic granule proteins, de novo lipid mediator generation, and cytokine release through vesicular transport. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

LILRA2: Does not bind class I MHC antigens. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to membrane; extracellular region

Molecular Function: receptor activity; antigen binding

Biological Process: immune system process; defense response; signal transduction