Rabbit anti-Human CD82 Polyclonal Antibody | anti-CD82 antibody

CD82 Antibody

Cat. Num. AAA9608668

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CD82; Polyclonal Antibody; CD82 Antibody; 4F9; Antigen detected by monoclonal and antibody IA4; C33; C33 antigen; CD 82; CD82 antigen; CD82 molecule; CD82_HUMAN; GR 15; GR15; IA 4; IA4; Inducible membrane protein; Inducible membrane protein R2; KAI 1; KAI1; Kangai 1 (suppression of tumorigenicity 6; prostate; CD82 antigen (R2 leukocyte antigen; antigen detected by monoclonal and antibody IA4)); Kangai 1; Kangai1; Leukocyte surface antigen R2; Metastasis suppressor Kangai 1; Metastasis suppressor Kangai-1; Metastasis suppressor Kangai1; Prostate cancer antimetastasis gene KAI1; R2; R2 leukocyte antigen; SAR 2; SAR2; ST 6; ST6; Suppression of tumorigenicity 6; Suppression of tumorigenicity 6 prostate; Suppressor of tumorigenicity 6; Suppressor of tumorigenicity 6 protein; Tetraspanin 27; Tetraspanin-27; Tetraspanin27; Tspan 27; Tspan-27; Tspan27; anti-CD82 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD82 antibody detects endogenous levels of total CD82
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 242

• Applicable Applications for anti-CD82 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human CD82
• Subcellular Location: Membrane.
• Tissue Specificity: Lymphoid specific.
• Predicted Cross Reactivity: Bovine
• Similarity: Bovine (80%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9608668 at 1/200 staining human lymph cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of CD82 using RAW264.7 whole cell lysates)

Related Product Information for anti-CD82 antibody

Function: Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Subunit Structure: Interacts directly with IGSF8.
Similarity: Belongs to the tetraspanin (TM4SF) family.

NCBI and Uniprot Product Information

• NCBI GI #: 67782354
• NCBI GeneID: 3732
• NCBI Accession #: NP_001020015.1
• NCBI GenBank Nucleotide #: NM_001024844.1
• UniProt Accession #: P27701; Q7Z2D4; Q7Z5N2; D3DQN6; E9PC70
• Molecular Weight: Observed: 30 kDa; Predicted: 30 kDa
• NCBI Official Full Name: CD82 antigen isoform 2
• NCBI Official Synonym Full Names: CD82 molecule
• NCBI Official Symbol: CD82
• NCBI Official Synonym Symbols: R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• NCBI Protein Information: CD82 antigen
• UniProt Protein Name: CD82 antigen
• Protein Family: CD82 antigen
• UniProt Gene Name: CD82
• UniProt Synonym Gene Names: KAI1; SAR2; ST6; TSPAN27; Tspan-27

NCBI Description

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.