Rabbit anti-Human CD70 Polyclonal Antibody | anti-CD70 antibody

CD70, ID (CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD70) (Azide free) (HRP)

Cat. Num. AAA6283416

• Gene Names: CD70; CD27L; CD27LG; TNFSF7
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD70; Polyclonal Antibody; ID (CD70; CD27L; CD27LG; TNFSF7; CD70 antigen; CD27 ligand; Tumor necrosis factor ligand superfamily member 7; CD70) (Azide free) (HRP); anti-CD70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD70.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD70 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 72-101 in the central region of human CD70.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NCI-H460 cell line lysates (35ug/lane) using 033534. This demonstrates that 033534 detected the CD70 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using 033534 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 033534 for immunohistochemistry.)

Related Product Information for anti-CD70 antibody

ptimal dilutions to be determined by the researcher.

Product Categories/Family for anti-CD70 antibody

Antibodies; Growth Factors; Cytokines

References

1. Arimoto-Miyamoto, K., et al. (2010) Immunology 130(1):137-149. 2. Shaw, J. et al.(2010) Blood 115(15):3051-3057. 3.Yu, S.E., et al. (2010)Mol. Cells 29(2):217-221. 4.0Kozlowska, A., et al. (2010)J. Rheumatol. 37(1):53-59. 5. Johnatty, S.E., et al. (2010)PLoS Genet. 6 (7), E1001016.

NCBI and Uniprot Product Information

• NCBI GI #: 4507605
• NCBI GeneID: 970
• NCBI Accession #: NP_001243.1
• NCBI GenBank Nucleotide #: NM_001252.3
• UniProt Accession #: P32970; Q96J57
• Molecular Weight: 21,118 Da
• NCBI Official Full Name: CD70 antigen
• NCBI Official Synonym Full Names: CD70 molecule
• NCBI Official Symbol: CD70
• NCBI Official Synonym Symbols: CD27L; CD27LG; TNFSF7
• NCBI Protein Information: CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
• UniProt Protein Name: CD70 antigen
• Protein Family: CD70 antigen
• UniProt Gene Name: CD70
• UniProt Synonym Gene Names: CD27L; CD27LG; TNFSF7; CD27-L
• UniProt Entry Name: CD70_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction