Rabbit anti-Human CD68 Polyclonal Antibody | anti-CD68 antibody

CD68, ID (DKFZp686M18236, GP110, Macrophage Antigen CD68, Macrosialin, Scavenger Receptor Class D Member 1, SCARD1) (AP)

Cat. Num. AAA6283368

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD68; Polyclonal Antibody; ID (DKFZp686M18236; GP110; Macrophage Antigen CD68; Macrosialin; Scavenger Receptor Class D Member 1; SCARD1) (AP); kpi; Gp110; anti-CD68 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD68.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CD68 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 209-237 in the central region of human CD68/CD68 (kpi),
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MDA-MB453 cell line lysates (35ug/lane) using 033530. This demonstrates the 033530 detected the CD68/CD68 (kpi) protein (arrow).)

Related Product Information for anti-CD68 antibody

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue-and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

Product Categories/Family for anti-CD68 antibody

Antibodies; Receptors; Scavenger

References

1. Leonarduzzi, G., et al. Mol Nutr Food Res 54 SUPPL 1, S31-S41 (2010). 2. Strojnik, T., et al. Anticancer Res. 29(8):3269-3279(2009). 3. Sayed, S., et al. Eur J Vasc Endovasc Surg 38(1):20-25(2009). 4. Suzuki, Y., et al. Int J Rheum Dis 12(1):7-13(2009). 5. Chen, W.S., et al. Scand. J. Rheumatol. 38(2):154-155(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 91199548
• NCBI GeneID: 968
• NCBI Accession #: NP_001242.2; NP_001035148.1
• NCBI GenBank Nucleotide #: NM_001251.2
• UniProt Accession #: P34810; Q53HR6; Q53XI3; Q96BI7; B4DVT4
• Molecular Weight: 34,654 Da
• NCBI Official Full Name: macrosialin isoform A
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

Uniprot Description

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane