Rabbit anti-Human CD50/ICAM-3 Polyclonal Antibody | anti-CD50/ICAM-3 antibody

CD50/ICAM-3 (Phospho-Ser518) Antibody

Cat. Num. AAA9508580

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: CD50/ICAM-3; Polyclonal Antibody; CD50/ICAM-3 (Phospho-Ser518) Antibody; CD50 antigen; CDw50; ICA3; ICAM-R; ICAM3; Intercellular adhesion molecule-3 precursor; anti-CD50/ICAM-3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: CD50/ICAM-3 (Phospho-Ser518) Antibody detects endogenous levels of ICAM3 only when phosphorylated at Ser518.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 547

• Applicable Applications for anti-CD50/ICAM-3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S518
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human CD50/ICAM-3 around the phosphorylation site of Ser518.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD50/ICAM-3 (Phospho-Ser518) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from HepG2 cells treated with TNF, using CD50/ICAM-3 (Phospho-Ser518) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NM_002162.4
• UniProt Accession #: P32942; Q6PD68
• Molecular Weight: 59 kDa
• NCBI Official Full Name: intercellular adhesion molecule 3 isoform 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3
• UniProt Protein Name: Intercellular adhesion molecule 3
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Uniprot Description

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).