Rabbit anti-Human CD5 Polyclonal Antibody | anti-CD5 antibody

CD5, ID (CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5) (AP)

Cat. Num. AAA6283324

• Gene Names: CD5; T1; LEU1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD5; Polyclonal Antibody; ID (CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5) (AP); anti-CD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CD5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-232 amino acids from the Central region of human CD5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CD5 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CD5 antibody detected the CD5 protein (arrow).)

Immunohistochemistry (IHC)

(CD5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CD5 antibody

CD5 may act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

Product Categories/Family for anti-CD5 antibody

Antibodies; CD Markers

References

Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Yoshino, N., et al. Ann Thorac Cardiovasc Surg 15(5):324-327(2009). Garaud, S., et al. Ann. N. Y. Acad. Sci. 1173, 280-285 (2009): Liang, X.S., et al. Br. J. Haematol. 146(4):418-423(2009). Vera, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(5):1506-1511(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NM_014207.3
• UniProt Accession #: P06127; A0N0P4; A8K9I3
• Molecular Weight: 54,578 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition