Rabbit CD47 Polyclonal Antibody | anti-CD47 antibody

Anti-CD47 Antibody

Cat. Num. AAA1751237

• Gene Names: CD47; IAP; OA3; MER6
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen Affinity Purified
• Synonyms: CD47; Polyclonal Antibody; Anti-CD47 Antibody; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; MER6; CD47 molecule; anti-CD47 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized
• Sequence Length: 292

• Applicable Applications for anti-CD47 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.5ug/ml; IHC-P: 0.5-1ug/ml; IHC-F: 0.5-1ug/ml; ICC/IF: 2ug/ml; FC/FACS: 1-3mug/1x106; Direct ELISA: 0.1-0.5ug/ml; Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.
• Immunogen: E Coli-derived human CD47 recombinant protein (Position: Q19-E323).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
• Relevant Detection Systems: We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P), IHC(F) and ICC.
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.; Avoid repeated freezing and thawing.

Related Product Information for anti-CD47 antibody

Description: Rabbit IgG polyclonal antibody for CD47 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

References

1. Bras, M., Yuste, V. J., Roue, G., Barbier, S., Sancho, P., Virely, C., Rubio, M., Baudet, S., Esquerda, J. E., Merle-Beral, H., Sarfati, M., Susin, S. A. Drp1 mediates caspase-independent type III cell death in normal and leukemic cells. Molec. Cell. Biol. 27: 7073-7088, 2007. 2. Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., Lindberg, F. P., Vignery, A. CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. J. Biol. Chem. 275: 37984-37992, 2000. 3. Oldenborg, P.-A., Zheleznyak, A., Fang, Y.-F., Lagenaur, C. F., Gresham, H. D., Lindberg, F. P. Role of CD47 as a marker of self on red blood cells. Science 288: 2051-2054, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 4502673
• NCBI GeneID: 961
• NCBI Accession #: NP_001768.1
• NCBI GenBank Nucleotide #: NM_001777.3
• UniProt Accession #: Q08722; Q53Y71; Q96A60; A8K198; D3DN59
• Molecular Weight: 31,743 Da
• NCBI Official Full Name: leukocyte surface antigen CD47 isoform 1
• NCBI Official Synonym Full Names: CD47 molecule
• NCBI Official Symbol: CD47
• NCBI Official Synonym Symbols: IAP; OA3; MER6
• NCBI Protein Information: leukocyte surface antigen CD47
• UniProt Protein Name: Leukocyte surface antigen CD47
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD47
• UniProt Synonym Gene Names: MER6; IAP

NCBI Description

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.