Rabbit anti-Human CD43 Polyclonal Antibody | anti-SPN antibody

Anti-CD43 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101175

• Gene Names: SPN; LSN; CD43; GALGP; GPL115
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD43; Polyclonal Antibody; Anti-CD43 Antibody; Rabbit Polyclonal; SPN/CD43 Antibody; Rabbit PAb; Antigen Affinity Purified; sialophorin; Anti-GALGP Antibody; Anti-GPL115 Antibody; Anti-LSN Antibody; anti-SPN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SPN/CD43
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SPN antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human SPN/CD43
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human SPN/CD43, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CD43 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 1 uL anti-CD43 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CD43 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size: 55 kDa)

Western Blot (WB)

(Anti-CD43 rabbit polyclonal antibody at 1:500 dilution Lane A: SKBR3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:59 kDa)

Related Product Information for anti-SPN antibody

CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. Besides, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.

References

Layseca-Espinosa E, et al. (2003) Journal of Leukocyte Biology. 74(6): 1083-93. Cannon JL, et al. (2011) Mol Biol Cell. 22(7):954-63. Pallant A, et al. (1989). Proc Natl Acad Sci. 86 (4): 1328-32.

NCBI and Uniprot Product Information

• NCBI GI #: 71979937
• NCBI GeneID: 6693
• NCBI Accession #: NP_001025459.1
• NCBI GenBank Nucleotide #: NM_001030288.2
• UniProt Accession #: P16150; A8K9B1
• Molecular Weight: 40,322 Da
• NCBI Official Full Name: leukosialin
• NCBI Official Synonym Full Names: sialophorin
• NCBI Official Symbol: SPN
• NCBI Official Synonym Symbols: LSN; CD43; GALGP; GPL115
• NCBI Protein Information: leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)
• UniProt Protein Name: Leukosialin
• Protein Family: Leukosialin
• UniProt Gene Name: SPN
• UniProt Synonym Gene Names: CD43; GALGP
• UniProt Entry Name: LEUK_HUMAN

NCBI Description

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Uniprot Description

sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; signal transduction; chemotaxis; negative thymic T cell selection; response to protozoan; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; blood coagulation; leukocyte migration