Rabbit CD43 Polyclonal Antibody | anti-SPN antibody

Anti-CD43 Antibody

Cat. Num. AAA176914

• Gene Names: SPN; LSN; CD43; GALGP; GPL115
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: CD43; Polyclonal Antibody; Anti-CD43 Antibody; Leukosialin; sialophorin; CD 43 antibody; CD43 antibody; CD43 antigen antibody; Galactoglycoprotein antibody; GALGP antibody; GPL 115 antibody; GPL115 antibody; Human gene for sialophorin antibody; Leucocyte sialoglycoprotein antibody; LEUK_HUMAN antibody; Leukocyte sialoglycoprotein antibody; Leukosialin antibody; LSN antibody; Sialophorin (leukosialin) antibody; Sialophorin antibody; SPN antibody; anti-SPN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized

• Applicable Applications for anti-SPN antibody: Western Blot (WB), Immunohistochemistry - Paraffin-embedded Section (IHC-P), Immunocytochemistry (ICC); ; Other applications have not been tested.
• Application Notes: WB: Concentration: 0.1-0.5ug/ml; Tested Species: Hu; Antigen Retrieval: -; ; IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Hu, Ms, Rat; Antigen Retrieval: By Heat; ; ICC: Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: -; ; WB: The detection limit for CD43 is approximately 0.25ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.; ; Optimal dilutions should be detrmined by end users.
• Immunogen: E Coli-derived human CD43 recombinant protein (Position: A272-P400). ; Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• Preparation and Storage: Reconstitution: 0.2ml of distilled water wil yield a concentration of 500ug/ml.; ; Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-CD43 Picoband antibody, MBS176914, Western blottingAll lanes: Anti CD43 (MBS176914) at 0.5ug/mlWB: Recombinant Human CD43 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD )

Immunohistochemistry (IHC)

(Anti-CD43 Picoband antibody, MBS176914, IHC(P)IHC(P): Mouse Spleen Tissue )

Immunohistochemistry (IHC)

(Anti-CD43 Picoband antibody, MBS176914, IHC(P)IHC(P): Rat Spleen Tissue )

Immunohistochemistry (IHC)

(Anti-CD43 Picoband antibody, MBS176914, IHC(P)IHC(P): Human Tonsil Tissue )

Immunocytochemistry (ICC)

(Anti-CD43 Picoband antibody, MBS176914, ICCICC: K562 Cell )

Related Product Information for anti-SPN antibody

CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

NCBI and Uniprot Product Information

• NCBI GI #: 126213
• NCBI GeneID: 6693
• UniProt Accession #: P16150; A8K9B1
• NCBI Official Full Name: Leukosialin
• NCBI Official Synonym Full Names: sialophorin
• NCBI Official Symbol: SPN
• NCBI Official Synonym Symbols: LSN; CD43; GALGP; GPL115
• NCBI Protein Information: leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)
• UniProt Protein Name: Leukosialin
• Protein Family: Leukosialin
• UniProt Gene Name: SPN
• UniProt Synonym Gene Names: CD43; GALGP
• UniProt Entry Name: LEUK_HUMAN

NCBI Description

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Uniprot Description

sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; chemotaxis; signal transduction; negative thymic T cell selection; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; response to protozoan; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of type IV hypersensitivity; leukocyte migration