Rabbit CD40 Polyclonal Antibody | anti-CD40 antibody

Rabbit anti CD40 Polyclonal Antibody

Cat. Num. AAA460187

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: CD40; Polyclonal Antibody; Rabbit anti CD40 Polyclonal Antibody; TNF recptor superfamily member 5; TNFRSF5; anti-CD40 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody recognizes human CD40 protein. It cross-reacts human, rat and mouse. The other species was not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 117

• Applicable Applications for anti-CD40 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-5 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml ; ; Optimal dilutions should be determined by researchers for specific applications.
• Antigen Preparation: A synthetic peptide QPVTQEDGKESRISVQERQ derived from human CD40 (258-277aa) was used as an immunogen.
• Positive Control: Spleen, Tonsil Tissue
• Cellular Location: Membrane/Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three months without detecable loss of activity. Avoid repeated freezing-thawing cycles.

Immunohistochemistry (IHC)

(Human tonsil tissue stained with Rabbit anti-CD40 (MBS460187) polyclonal antibody at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20min.)

Related Product Information for anti-CD40 antibody

The CD40 is a member of the TNF-receptor superfamily. It is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad varity of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adapter protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). CD40 is a B cell surface antigen.

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• NCBI GeneID: 958
• Molecular Weight: ~35 kDa
• NCBI Official Full Name: CD40, partial
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: enzyme binding; protein binding; receptor activity; signal transducer activity; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; immune response; inflammatory response; multicellular organismal development; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; protein complex assembly; regulation of cell proliferation; regulation of immune response; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency With Hyper-igm, Type 3