Western Blot (WB)
(Western blot analysis of extracts ofvarious cell lines, using SIGLEC3/CD33 antibody (MBS127495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Rabbit CD33 Polyclonal Antibody | anti-CD33 antibody
CD33 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts ofvarious cell lines, using SIGLEC3/CD33 antibody (MBS127495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts ofvarious cell lines, using SIGLEC3/CD33 antibody (MBS127495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Immunohistochemistry
(Immunohistochemistry of paraffin-embedded mouse spleen using SIGLEC3/CD33 antibody (MBS127495) atdilution of 1:200 (40x lens).)
Immunohistochemistry
(Immunohistochemistry of paraffin-embedded mouse spleen using SIGLEC3/CD33 antibody (MBS127495) atdilution of 1:200 (40x lens).)
Immunofluorescence
(Immunofluorescence analysis of THP-1 cells using CD33 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence
(Immunofluorescence analysis of THP-1 cells using CD33 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Observed MW: 77kDa
Uniprot Description
CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity; carbohydrate binding
Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion
Research Articles on CD33
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Product Notes
The CD33 cd33 (Catalog #AAA127495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD33 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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