Goat CD274 Polyclonal Antibody | anti-CD274 antibody

GOAT ANTI HUMAN CD274 (C-TERMINAL)

Cat. Num. AAA223738

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: CD274; Polyclonal Antibody; GOAT ANTI HUMAN CD274 (C-TERMINAL); anti-CD274 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons. ; ; CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. ; ; Studies have linked the deregulation of CD274 function with many autoimmune diseases and cancers, as well as chronic viral and intracellular bacterial infection.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 176

• Applicable Applications for anti-CD274 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 35kDa in human heart cell lysates.; ELISA: Minimum Dilution: 1/8000; Immunohistology - Paraffin: Minimum Dilution: 4.0; Maximum Dilution: 6.0ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.3; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1).; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Paraffin embeded human skeletal muscle stained with Goat anti Human CD274 C-Terminal (MBS223738))

Testing Data #2

(Human heart lysate probed with Goat anti Human CD274 C-Terminal (MBS223738))

Related Product Information for anti-CD274 antibody

MBS223738 specifically recognises an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons. CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. Studies have linked the deregulation of CD274 function with many autoimmune diseases and cancers, as well as chronic viral and intracellular bacterial infection.

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NM_001267706.1
• UniProt Accession #: Q9NZQ7; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
• Molecular Weight: 20,454 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• Protein Family: Programmed cell death
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction