Rabbit anti-Human CD1B Polyclonal Antibody | anti-CD1B antibody

CD1B, NT (CD1B, T-cell surface glycoprotein CD1b, CD1b) (Azide free) (HRP)

Cat. Num. AAA6282865

• Gene Names: CD1B; R1; CD1; CD1A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD1B; Polyclonal Antibody; NT (CD1B; T-cell surface glycoprotein CD1b; CD1b) (Azide free) (HRP); CD1b; anti-CD1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CD1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human CD1B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CD1B (arrow) using rabbit polyclonal CD1B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD1B gene.)

Related Product Information for anti-CD1B antibody

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq].

Product Categories/Family for anti-CD1B antibody

Antibodies; CD Markers

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Felio, K., et al. J. Exp. Med. 206(11):2497-2509(2009). Guiard, J., et al. J. Immunol. 182(11):7030-7037(2009). Cui, Y., et al. Biol. Direct 4, 47 (2009): Zajonc, D.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(46):17925-17930(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4502645
• NCBI GeneID: 910
• NCBI Accession #: NP_001755.1
• NCBI GenBank Nucleotide #: NM_001764.2
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2
• Molecular Weight: 30,740 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1b
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1b; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1B
• UniProt Entry Name: CD1B_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response