Goat anti-Yeast CD154 Polyclonal Antibody | anti-CD154 antibody

GOAT ANTI HUMAN CD154

Cat. Num. AAA221334

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Reactivity: Yeast
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: CD154; Polyclonal Antibody; GOAT ANTI HUMAN CD154; anti-CD154 antibody

• Host: Goat
• Reactivity: Yeast
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-CD154 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Purified goat anti human CD154 may be used in an indirect ELISA or as a capture reagent in a sadwich ELISA with our biotinylated human CD154 antibody as the detection reagent and recombinant human CD154 as the standard.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Immunohistology - Paraffin: Minimum Dilution: 2.5; Maximum Dilution: 5.0ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Immunogen: Recombinant human CD154; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human CD154 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Goat anti Human CD154 (MBS221334))

Testing Data #2

(Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Goat anti Human CD154 (MBS221334))

Related Product Information for anti-CD154 antibody

Goat anti Human CD154 antibody recognizes the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154. CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI Accession #: NP_000065.1
• NCBI GenBank Nucleotide #: NM_000074.2
• UniProt Accession #: P29965
• Molecular Weight: 29,274 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN

NCBI Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1