Rabbit anti-Human CD13 Polyclonal Antibody | anti-CD13 antibody

CD13, ID (Aminopeptidase N, AP-N, hAPN, Alanyl Aminopeptidase, Microsomal Aminopeptidase, Aminopeptidase M, AP-M, gp150, Myeloid Plasma Membrane Glycoprotein CD13, ANPEP, PEPN, APN) (Biotin)

Cat. Num. AAA6468008

• Gene Names: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CD13; Polyclonal Antibody; ID (Aminopeptidase N; AP-N; hAPN; Alanyl Aminopeptidase; Microsomal Aminopeptidase; Aminopeptidase M; AP-M; gp150; Myeloid Plasma Membrane Glycoprotein CD13; ANPEP; PEPN; APN) (Biotin); anti-CD13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANPEP.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CD13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ANPEP (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ANPEP antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). ANPEP (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CD13 antibody

Antibodies; CD Markers

References

1. Watt, V.M., Willard, H.F. Hum. Genet. 85(6), 651-654 (1990). 2. O'Connell, P.J., Gerkis, V. J. Biol. Chem. 266(7), 4593-4597 (1991). 3. Shapiro, L.H., Ashmun, R.A. J. Biol. Chem. 266(18), 11999-12007 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 157266300
• NCBI GeneID: 290
• NCBI Accession #: NP_001141
• NCBI GenBank Nucleotide #: NM_001150
• UniProt Accession #: P15144; Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0
• Molecular Weight: 109,540 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; CD13; LAP1; P150; PEPN; GP150
• NCBI Protein Information: aminopeptidase N; AP-M; AP-N; alanyl aminopeptidase; aminopeptidase M; hAPN; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APN; CD13; PEPN; AP-N; hAPN; AP-M
• UniProt Entry Name: AMPN_HUMAN

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Receptor, misc.; EC 3.4.11.2; Other Amino Acids Metabolism - glutathione; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 15q25-q26

Cellular Component: extracellular space; lysosomal membrane; integral to plasma membrane; ER-Golgi intermediate compartment; cytosol; external side of plasma membrane

Molecular Function: viral receptor activity; zinc ion binding; metallopeptidase activity; receptor activity; aminopeptidase activity

Biological Process: entry of virus into host cell; cellular protein metabolic process; angiogenesis; angiotensin maturation; cell differentiation; proteolysis