Rabbit CD10 Polyclonal Antibody | anti-CD10 antibody

CD10 Antibody

Cat. Num. AAA9410562

• Gene Names: MME; NEP; SFE; CD10; CALLA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: CD10; Polyclonal Antibody; CD10 Antibody; NEP; MME; SFE; CALLA; MGC126681; anti-CD10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CD10 protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 750

• Applicable Applications for anti-CD10 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa.6~10(S-Q-M-D-I) derived from Human CD10.
• Target Name: CD10
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extract from Rat kidney tissue using CD10 Antibody)

Related Product Information for anti-CD10 antibody

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Product Categories/Family for anti-CD10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85,514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo-endopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: CD109 antigen
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_HUMAN

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: Protease; EC 3.4.24.11; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: synaptic vesicle; focal adhesion; integral to plasma membrane; axon; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: peptide metabolic process; cellular protein metabolic process; beta-amyloid metabolic process; creatinine metabolic process; proteolysis; sensory perception of pain; kidney development; angiotensin maturation

Disease: Membranous Nephropathy, Susceptibility To