Immunofluorescence (IF)
(Immunofluorescence staining of CCR1/CD191 in HESS9 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CCR1/CD191 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cell membrane.)
Rabbit anti-Human CCR1 Polyclonal Antibody | anti-CCR1 antibody
Anti-CCR1 Antibody, Rabbit Polyclonal
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate
Immunofluorescence (IF)
(Immunofluorescence staining of CCR1/CD191 in HESS9 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CCR1/CD191 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of CCR1/CD191 in HESS9 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CCR1/CD191 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cell membrane.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CCR1/CD191 in human tonsil with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CCR1/CD191 in human tonsil with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CCR1/CD191 in human spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CCR1/CD191 in human spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(CCR1/CD191 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-CCR1/CD191 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CCR1/CD191 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
Immunoprecipitation (IP)
(CCR1/CD191 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-CCR1/CD191 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CCR1/CD191 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
Western Blot (WB)
(Anti-CCR1/CD191 rabbit polyclonal antibody at 1:500 dilution.Lane A: HEK293 Membrane LysateLane B: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa)
Western Blot (WB)
(Anti-CCR1/CD191 rabbit polyclonal antibody at 1:500 dilution.Lane A: HEK293 Membrane LysateLane B: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
Research Articles on CCR1
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Product Notes
The CCR1 ccr1 (Catalog #AAA8100373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCR1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:2000 ICC/IF: 1:300-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the CCR1 ccr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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