Rabbit anti-Human, Mouse CCNT1 Polyclonal Antibody | anti-CCNT1 antibody

CCNT1, ID (CCNT1, Cyclin-T1) (MaxLight 750)

Cat. Num. AAA6282551

• Gene Names: CCNT1; CCNT; CYCT1; HIVE1
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CCNT1; Polyclonal Antibody; ID (CCNT1; Cyclin-T1) (MaxLight 750); Cyclin-T1; anti-CCNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCNT1. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-CCNT1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 253-281 in the central region of human CCNT1.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6003220. This demonstrates MBS6003220 detected the CCNT1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6003220. This demonstrates MBS6003220 detected the CCNT1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MCF-7 cells using MBS6003220 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCNT1 antibody

CCNT1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

Product Categories/Family for anti-CCNT1 antibody

Antibodies; Cyclin

References

Moiola, C., et al. Cell Cycle 9(15):3119-3126(2010). Schonichen, A., et al. Biochemistry 49(14):3083-3091(2010). Czudnochowski, N., et al. J. Mol. Biol. 395(1):28-41(2010). Kapasi, A.J., et al. J. Virol. 83(11):5904-5917(2009). Cho, S., et al. EMBO J. 28(10):1407-1417(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17978466
• NCBI GeneID: 904
• NCBI Accession #: NP_001231
• NCBI GenBank Nucleotide #: NM_001240.3
• UniProt Accession #: O60563; O60581; A9XU13; E7EX76
• Molecular Weight: Predicted: 80 kDa
• NCBI Official Full Name: cyclin-T1 isoform a
• NCBI Official Synonym Full Names: cyclin T1
• NCBI Official Symbol: CCNT1
• NCBI Official Synonym Symbols: CCNT; CYCT1; HIVE1
• NCBI Protein Information: cyclin-T1; cyclin C-related protein; CDK9-associated C-type protein; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
• UniProt Protein Name: Cyclin-T1
• Protein Family: Cyclin
• UniProt Gene Name: CCNT1
• UniProt Synonym Gene Names: CycT1; Cyclin-T
• UniProt Entry Name: CCNT1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CCNT1: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC. Ubiquitously expressed. Belongs to the cyclin family. Cyclin C subfamily.

Protein type: Cell cycle regulation; Transcription initiation complex

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; transcription elongation factor complex b; nucleolus; nucleus

Molecular Function: protein binding; snRNA binding; DNA binding; chromatin binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; cell cycle; protein amino acid phosphorylation; cell division; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity