Rabbit anti-Human CCNDBP1 Polyclonal Antibody | anti-CCNDBP1 antibody

CCNDBP1, NT (CCNDBP1, DIP1, GCIP, HHM, Cyclin-D1-binding protein 1, Grap2 and cyclin-D-interacting protein, Human homolog of Maid) (PE)

Cat. Num. AAA6282387

• Gene Names: CCNDBP1; HHM; DIP1; GCIP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCNDBP1; Polyclonal Antibody; NT (CCNDBP1; DIP1; GCIP; HHM; Cyclin-D1-binding protein 1; Grap2 and cyclin-D-interacting protein; Human homolog of Maid) (PE); Human homolog of Maid; anti-CCNDBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CCNDBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCNDBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human CCNDBP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CCNDBP1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCNDBP1 antibody detected the CCNDBP1 protein (arrow).)

Related Product Information for anti-CCNDBP1 antibody

This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-CCNDBP1 antibody

Antibodies; Cyclin

References

Lee, I., et al. Cancer Res. 70(11):4357-4365(2010). Seto, A., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 (PT 1), 21-24 (2009): Ikushima, H., et al. EMBO J. 27(22):2955-2965(2008). Chen, W.C., et al. Histopathology 53(5):554-560(2008). Chang, T.W., et al. Oncogene 27(3):332-338(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 227500367
• NCBI GeneID: 23582
• NCBI Accession #: NP_036274.3
• NCBI GenBank Nucleotide #: NM_012142.4
• UniProt Accession #: O95273; Q6ZQN9; Q7Z519; Q8NBS7; Q8NBY2; Q9NS19; Q9NYH3; Q9UHX9; A8K3Q0; A8K3U2
• Molecular Weight: 22,714 Da
• NCBI Official Full Name: cyclin-D1-binding protein 1
• NCBI Official Synonym Full Names: cyclin D-type binding-protein 1
• NCBI Official Symbol: CCNDBP1
• NCBI Official Synonym Symbols: HHM; DIP1; GCIP
• NCBI Protein Information: cyclin-D1-binding protein 1; human homolog of Maid; D-type cyclin-interacting protein 1; grap2 and cyclin-D-interacting protein
• UniProt Protein Name: Cyclin-D1-binding protein 1
• Protein Family: Cyclin-D1-binding protein
• UniProt Gene Name: CCNDBP1
• UniProt Synonym Gene Names: DIP1; GCIP; HHM
• UniProt Entry Name: CCDB1_HUMAN

NCBI Description

This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

CCNDBP1: May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F- dependent transcription. Belongs to the CCNDBP1 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q14-q15

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: regulation of cell cycle; cell cycle