Rabbit anti-Human CCND2 / Cyclin D2 Polyclonal Antibody | anti-CCND2 antibody

Anti-CCND2 / Cyclin D2 Antibody (aa1-30) IHC-plus

Cat. Num. AAA249853

• Gene Names: CCND2; MPPH3; KIAK0002
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: CCND2 / Cyclin D2; Polyclonal Antibody; Anti-CCND2 / Cyclin D2 Antibody (aa1-30) IHC-plus; Rabbit Polyclonal to Human CCND2 / Cyclin D2; Human CCND2 / Cyclin D2; KIAK0002; G1/S-specific cyclin D2; G1/S-specific cyclin-D2; Cyclin D2; anti-CCND2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human Cyclin D2
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 289

• Applicable Applications for anti-CCND2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:50); WB (1:1000)
• Immunogen: CCND2 / Cyclin D2 antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human CCND2.
• Immunogen Description: KLH-conjugated synthetic peptide from N-terminal region of human CCND2.
• Immunogen Type: Synthetic peptide
• Antigen Modification: aa1-30
• Target Species: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-CCND2 / Cyclin D2 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(Western blot of CCND2 (arrow) using rabbit polyclonal CCND2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CCND2 gene.)

Western Blot (WB)

(CCND2 Antibody western blot of CEM cell line lysates (35 ug/lane). The CCND2 antibody detected the CCND2 protein (arrow).)

Related Product Information for anti-CCND2 antibody

CCND2 Antibody, KIAK0002 Antibody, G1/S-specific cyclin D2 Antibody, G1/S-specific cyclin-D2 Antibody, Cyclin D2 Antibody Description: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.

NCBI and Uniprot Product Information

• NCBI GI #: 4502617
• NCBI GeneID: 894
• NCBI Accession #: NP_001750.1
• NCBI GenBank Nucleotide #: NM_001759.3
• UniProt Accession #: P30279; Q13955; Q5U035; A8K531
• Molecular Weight: 24,461 Da
• NCBI Official Full Name: G1/S-specific cyclin-D2
• NCBI Official Synonym Full Names: cyclin D2
• NCBI Official Symbol: CCND2
• NCBI Official Synonym Symbols: MPPH3; KIAK0002
• NCBI Protein Information: G1/S-specific cyclin-D2
• UniProt Protein Name: G1/S-specific cyclin-D2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND2
• UniProt Entry Name: CCND2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]

Uniprot Description

CCND2: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Belongs to the cyclin family. Cyclin D subfamily.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: cell cycle; cell division; positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 3