Rabbit anti-Human CCND2 Polyclonal Antibody | anti-CCND2 antibody

CCND2 (Cyclin D2, KIAK0002, MGC102758) (Biotin)

Cat. Num. AAA6409976

• Gene Names: CCND2; KIAK0002
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: CCND2; Polyclonal Antibody; CCND2 (Cyclin D2; KIAK0002; MGC102758) (Biotin); anti-CCND2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCND2.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CCND2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human CCND2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of 231 cell line, using CCND2 antibody.)

Product Categories/Family for anti-CCND2 antibody

Antibodies; Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 4502617
• NCBI GeneID: 894
• NCBI Accession #: NP_001750.1
• NCBI GenBank Nucleotide #: NM_001759.3
• UniProt Accession #: P30279; Q13955; Q5U035; A8K531
• Molecular Weight: 33,067 Da
• NCBI Official Full Name: G1/S-specific cyclin-D2
• NCBI Official Synonym Full Names: cyclin D2
• NCBI Official Symbol: CCND2
• NCBI Official Synonym Symbols: KIAK0002
• NCBI Protein Information: G1/S-specific cyclin-D2; G1/S-specific cyclin D2
• UniProt Protein Name: G1/S-specific cyclin-D2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND2
• UniProt Entry Name: CCND2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. [provided by RefSeq, Oct 2008]

Uniprot Description

CCND2: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Belongs to the cyclin family. Cyclin D subfamily.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleoplasm; nuclear membrane; cyclin-dependent protein kinase holoenzyme complex; nucleolus; cytosol; chromatin; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of protein amino acid phosphorylation; cell cycle

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 3