Rabbit anti-Human, Mouse CCND1 / Cyclin D1 Polyclonal Antibody | anti-CCND1 antibody

Rabbit Polyclonal to Human CCND1 / Cyclin D1

Cat. Num. AAA242546

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CCND1 / Cyclin D1; Polyclonal Antibody; Rabbit Polyclonal to Human CCND1 / Cyclin D1; Anti-CCND1 / Cyclin D1 Antibody (Ser90) IHC-plus; CCND1; B-cell lymphoma 1 protein; BCL-1 oncogene; B-cell CLL/lymphoma 1; BCL-1; Cyclin D1; G1/S-specific cyclin-D1; U21B31; PRAD1; BCL1; D11S287E; PRAD1 oncogene; Human CCND1; anti-CCND1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Concentration: 0.25 mg/ml (varies by lot)
• Sequence Length: 295

• Applicable Applications for anti-CCND1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.
• Immunogen Type: Synthetic peptide
• Immunogen: CCND1 / Cyclin D1 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.
• Antigen Modification: Ser90
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescence of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Western Blot (WB)

(Western blot of mouse lung and bladder tissue lysates (35 ug/lane).)

Western Blot (WB)

(Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified Pab.)

Related Product Information for anti-CCND1 antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.

NCBI and Uniprot Product Information

• NCBI GI #: 16950655
• NCBI GeneID: 595
• NCBI Accession #: NP_444284.1
• NCBI GenBank Nucleotide #: NM_053056.2
• UniProt Accession #: P24385; Q6LEF0
• Molecular Weight: 33,729 Da
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1; BCL-1 oncogene; PRAD1 oncogene; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein
• UniProt Protein Name: G1/S-specific cyclin-D1
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND1
• UniProt Synonym Gene Names: BCL1; PRAD1; BCL-1
• UniProt Entry Name: CCND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Activator; Oncoprotein; Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; transcriptional repressor complex; membrane; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription corepressor activity; protein kinase binding; transcription factor binding; protein kinase activity

Biological Process: fat cell differentiation; G1 DNA damage checkpoint; lactation; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; re-entry into mitotic cell cycle; negative regulation of Wnt receptor signaling pathway; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; liver development; response to organic nitrogen; response to ethanol; response to estrogen stimulus; cell division; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple