Rabbit CCND1 Polyclonal Antibody | anti-CCND1 antibody

Rabbit Anti Human CCND1 PolyClonal Antibody

Cat. Num. AAA1490299

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: CCND1; Polyclonal Antibody; Rabbit Anti Human CCND1 PolyClonal Antibody; BCL1; D11S287E; PRAD1; U21B31; G1/S-specific cyclin-D1; BCL-1 oncogenePRAD1 oncogene; anti-CCND1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 295

• Applicable Applications for anti-CCND1 antibody: Western Blot (WB) , Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen: A synthetic peptide of Human Cyclin D1
• Relevance: Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation.
• Storage Buffer: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

NCBI and Uniprot Product Information

• NCBI GI #: 49457151
• NCBI GeneID: 595
• UniProt Accession #: Q6FI00; P24385
• Molecular Weight: 33,729 Da
• NCBI Official Full Name: CCND1, partial
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1; BCL-1 oncogene; PRAD1 oncogene; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein
• UniProt Protein Name: CCND1 protein
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND1
• UniProt Entry Name: Q6FI00_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Activator; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription corepressor activity; transcription factor binding; protein kinase binding; protein kinase activity

Biological Process: fat cell differentiation; lactation; G1 DNA damage checkpoint; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; re-entry into mitotic cell cycle; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to vitamin E; response to magnesium ion; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; organ regeneration; Notch signaling pathway; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; response to estrogen stimulus; cell division; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple