Rabbit anti-Human CCNB3 Polyclonal Antibody | anti-CCNB3 antibody

CCNB3, Phosphorylated (T280) (CCNB3, CYCB3, G2/mitotic-specific cyclin-B3) (AP)

Cat. Num. AAA6282300

• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCNB3; Polyclonal Antibody; Phosphorylated (T280) (CCNB3; CYCB3; G2/mitotic-specific cyclin-B3) (AP); G2/mitotic-specific cyclin-B3; anti-CCNB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CCNB3 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T280 of human CCNB3.
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of CCNB3 Antibody (Phospho T280) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-CCNB3 antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chick and Drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CCNB3 antibody

Antibodies; Cyclin

References

Cheng, J., et al. Science 308(5725):1149-1154(2005). Nguyen, T.B., et al. J. Biol. Chem. 277(44):41960-41969(2002). Hrimech, M., et al. EMBO J. 21 (14), 3918 (2002): Lozano, J.C., et al. Biochem. Biophys. Res. Commun. 291(2):406-413(2002). Mouland, A.J., et al. Virology 292(2):321-330(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 90669307
• NCBI GeneID: 85417
• NCBI Accession #: NP_149020.2
• NCBI GenBank Nucleotide #: NM_033031.2
• UniProt Accession #: Q8WWL7; Q96SB5; Q96SB6; Q96SB7; Q9NT38; B1AQI5; B1AQI6
• Molecular Weight: 12,195 Da
• NCBI Official Full Name: G2/mitotic-specific cyclin-B3 isoform 3
• NCBI Official Synonym Full Names: cyclin B3
• NCBI Official Symbol: CCNB3
• NCBI Protein Information: G2/mitotic-specific cyclin-B3
• UniProt Protein Name: G2/mitotic-specific cyclin-B3
• UniProt Gene Name: CCNB3
• UniProt Synonym Gene Names: CYCB3
• UniProt Entry Name: CCNB3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as positive regulators of cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chicken and drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and may be required for proper spindle reorganization and restoration of the interphase nucleus. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]