Goat CCL20 Polyclonal Antibody | anti-CCL20 antibody

Goat anti-CCL20 Antibody

Cat. Num. AAA421719

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow, Pig
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CCL20; Polyclonal Antibody; Goat anti-CCL20 Antibody; chemokine (C-C motif) ligand 20; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; anti-CCL20 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)

• Applicable Applications for anti-CCL20 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Immunohistochemistry: In paraffin embedded Human Liver shows local staining between the hepatocytes. Recommended concentration, 6-8ug/ml.; Western Blot: Preliminary experiments in Human Peripheral Blood Mononucleocytes lysates gave no specific signal but low background (at antibody concentration up to 2ug/ml).
• Immunogen: Peptide with sequence CLGYTDRILHPK, from the internal region of the protein sequence according to NP_004582.1; NP_001123518.1.
• Note: This antibody is expected to recognize both isoforms (NP_004582.1; NP_001123518.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4759076
• NCBI GeneID: 6364
• NCBI Accession #: NP_004582.1; NP_001123518.1
• NCBI GenBank Nucleotide #: NM_004591.2
• NCBI Official Full Name: C-C motif chemokine 20 isoform 1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; protein binding

Biological Process: cell-cell signaling; chemotaxis; defense response to bacterium; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; signal transduction; wound healing